Ultrafast myoglobin structural dynamics observed with an X-ray free-electron laser

Levantino, Matteo; Schirò, Giorgio; Lemke, Henrik Till; Cottone, Grazia; Glownia, James Michael; Zhu, Diling; Chollet, Mathieu; Ihee, Hyotcherl; KAIST, Daejeon; Cupane, Antonio; Cammarata, Marco

doi:10.1038/ncomms7772

Title: Ultrafast myoglobin structural dynamics observed with an X-ray free-electron laser

Journal Article · Thu Apr 02 00:00:00 EDT 2015 · Nature Communications

DOI:https://doi.org/10.1038/ncomms7772· OSTI ID:1189945

Levantino, Matteo ^[1]; Schirò, Giorgio ^[2]; Lemke, Henrik Till ^[3]; Cottone, Grazia ^[1]; Glownia, James Michael ^[3]; Zhu, Diling ^[3]; Chollet, Mathieu ^[3]; Ihee, Hyotcherl ^[4]; KAIST, Daejeon ^[5]; Cupane, Antonio ^[1]; Cammarata, Marco ^[6]

Univ. of Palermo (Italy). Dept. of Physics.
Univ. of Grenoble Alpes and Institute of Structural Biology (France)
SLAC National Accelerator Laboratory, Menlo Park, CA (Untied States)
Inst. for Basic Science (IBS), Daejeon (Republic of Korea)
Republic of Korea
Univ. of Rennes (France). Dept. of Physics.

Light absorption can trigger biologically relevant protein conformational changes. The light induced structural rearrangement at the level of a photoexcited chromophore is known to occur in the femtosecond timescale and is expected to propagate through the protein as a quake-like intramolecular motion. Here we report direct experimental evidence of such ‘proteinquake’ observed in myoglobin through femtosecond X-ray solution scattering measurements performed at the Linac Coherent Light Source X-ray free-electron laser. An ultrafast increase of myoglobin radius of gyration occurs within 1 picosecond and is followed by a delayed protein expansion. As the system approaches equilibrium it undergoes damped oscillations with a ~3.6-picosecond time period. Our results unambiguously show how initially localized chemical changes can propagate at the level of the global protein conformation in the picosecond timescale.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

OSTI ID:: 1189945

Journal Information:: Nature Communications, Vol. 6, Issue 3; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 134 works

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