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Title: Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis

Abstract

Bacterial extracellular metal respiration, as carried out by members of the genus Geobacter, is of interest for applications including microbial fuel cells and bioremediation. Geobacter bemidjiensis is the major species whose growth is stimulated during groundwater amendment with acetate. We have carried out label-free proteomics studies of Geobacter bemidjiensis grown with acetate as the electron donor and either fumarate, ferric citrate, or one of two hydrous ferric oxide mineral types as electron acceptor. The major class of proteins whose expression changes across these conditions is c-type cytochromes, many of which are known to be involved in extracellular metal reduction in other, better-characterized Geobacter species. Some proteins with multiple homologues in G. bemidjiensis (OmcS, OmcB) had different expression patterns than observed for their G. sulfurreducens homologues under similar growth conditions. We also compared the proteome from our study to a prior proteomics study of biomass recovered from an aquifer in Colorado, where the microbial community was dominated by strains closely-related to G. bemidjiensis. We detected an increased number of proteins with functions related to motility and chemotaxis in the Colorado field samples compared to the laboratory samples, suggesting the importance of motility for in situ extracellular metal respiration.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1188889
Report Number(s):
PNNL-SA-104781
18427; 48680; KP1601010
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Proteome Research, 14(3):1361-1375
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Merkley, Eric D., Wrighton, Kelly C., Castelle, Cindy, Anderson, Brian J., Wilkins, Michael J., Shah, Vega, Arbour, Tyler, Brown, Joseph N., Singer, Steven W., Smith, Richard D., and Lipton, Mary S.. Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis. United States: N. p., 2015. Web. doi:10.1021/pr500983v.
Merkley, Eric D., Wrighton, Kelly C., Castelle, Cindy, Anderson, Brian J., Wilkins, Michael J., Shah, Vega, Arbour, Tyler, Brown, Joseph N., Singer, Steven W., Smith, Richard D., & Lipton, Mary S.. Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis. United States. doi:10.1021/pr500983v.
Merkley, Eric D., Wrighton, Kelly C., Castelle, Cindy, Anderson, Brian J., Wilkins, Michael J., Shah, Vega, Arbour, Tyler, Brown, Joseph N., Singer, Steven W., Smith, Richard D., and Lipton, Mary S.. Fri . "Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis". United States. doi:10.1021/pr500983v.
@article{osti_1188889,
title = {Changes in protein expression across laboratory and field experiments in Geobacter bemidjiensis},
author = {Merkley, Eric D. and Wrighton, Kelly C. and Castelle, Cindy and Anderson, Brian J. and Wilkins, Michael J. and Shah, Vega and Arbour, Tyler and Brown, Joseph N. and Singer, Steven W. and Smith, Richard D. and Lipton, Mary S.},
abstractNote = {Bacterial extracellular metal respiration, as carried out by members of the genus Geobacter, is of interest for applications including microbial fuel cells and bioremediation. Geobacter bemidjiensis is the major species whose growth is stimulated during groundwater amendment with acetate. We have carried out label-free proteomics studies of Geobacter bemidjiensis grown with acetate as the electron donor and either fumarate, ferric citrate, or one of two hydrous ferric oxide mineral types as electron acceptor. The major class of proteins whose expression changes across these conditions is c-type cytochromes, many of which are known to be involved in extracellular metal reduction in other, better-characterized Geobacter species. Some proteins with multiple homologues in G. bemidjiensis (OmcS, OmcB) had different expression patterns than observed for their G. sulfurreducens homologues under similar growth conditions. We also compared the proteome from our study to a prior proteomics study of biomass recovered from an aquifer in Colorado, where the microbial community was dominated by strains closely-related to G. bemidjiensis. We detected an increased number of proteins with functions related to motility and chemotaxis in the Colorado field samples compared to the laboratory samples, suggesting the importance of motility for in situ extracellular metal respiration.},
doi = {10.1021/pr500983v},
journal = {Journal of Proteome Research, 14(3):1361-1375},
number = ,
volume = ,
place = {United States},
year = {Fri Mar 06 00:00:00 EST 2015},
month = {Fri Mar 06 00:00:00 EST 2015}
}