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Title: Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC–ESI-MS–MS

Journal Article · · Analytical and Bioanalytical Chemistry
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  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States)
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Described here are the results from the profiling of the proteins arginine vasopressin (AVP) and adrenocorticotropic hormone (ACTH) from normal human pituitary gland and pituitary adenoma tissue sections using a fully automated droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system for spatially resolved sampling, HPLC separation, and mass spectral detection. Excellent correlation was found between the protein distribution data obtained with this droplet-based liquid microjunction surface sampling-HPLC-ESI-MS/MS system and those data obtained with matrix assisted laser desorption ionization (MALDI) chemical imaging analyses of serial sections of the same tissue. The protein distributions correlated with the visible anatomic pattern of the pituitary gland. AVP was most abundant in the posterior pituitary gland region (neurohypophysis) and ATCH was dominant in the anterior pituitary gland region (adenohypophysis). The relative amounts of AVP and ACTH sampled from a series of ACTH secreting and non-secreting pituitary adenomas correlated with histopathological evaluation. ACTH was readily detected at significantly higher levels in regions of ACTH secreting adenomas and in normal anterior adenohypophysis compared to non-secreting adenoma and neurohypophysis. AVP was mostly detected in normal neurohypophysis as anticipated. This work demonstrates that a fully automated droplet-based liquid microjunction surface sampling system coupled to HPLC-ESI-MS/MS can be readily used for spatially resolved sampling, separation, detection, and semi-quantitation of physiologically-relevant peptide and protein hormones, such as AVP and ACTH, directly from human tissue. In addition, the relative simplicity, rapidity and specificity of the current methodology support the potential of this basic technology with further advancement for assisting surgical decision-making.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
Work for Others (WFO)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1186003
Journal Information:
Analytical and Bioanalytical Chemistry, Vol. 407, Issue 20; ISSN 1618-2642
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 24 works
Citation information provided by
Web of Science

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Cited By (5)

Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions journal February 2018
A target-driven DNA-based molecular machine for rapid and homogeneous detection of arginine-vasopressin journal January 2020
Metabolomics—A Promising Approach to Pituitary Adenomas journal January 2019
Quantitative biodistribution of biotherapeutics at whole body, organ and cellular levels by autoradiography journal September 2018
Spatially resolved absolute quantitation in thin tissue by mass spectrometry journal November 2020

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
chemical imaging
liquid microjunction
droplet-based liquid extraction
autosampler
spatial distribution
human pituitary
protein
pituitary adenoma