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Title: Development of Agave as a dedicated biomass source: production of biofuels from whole plants

Abstract

Background: Agave species can grow well in semi-arid marginal agricultural lands around the world. Selected Agave species are used largely for alcoholic beverage production in Mexico. There are expanding research efforts to use the plentiful residues (bagasse) for ethanol production as the beverage manufacturing process only uses the juice from the central core of mature plants. Here we investigate the potential of over a dozen Agave species, including three from cold semi-arid regions of the United States, to produce biofuels using the whole plant. Results: Ethanol was readily produced by Saccharomyces cerevisiae from hydrolysate of ten whole Agaves with the use of a proper blend of biomass degrading enzymes that overcomes toxicity of most of the species tested. Unlike yeast fermentations, Clostridium beijerinckii produced butanol plus acetone from nine species tested. Butyric acid, a precursor of butanol, was also present due to incomplete conversion during the screening process. Since Agave contains high levels of free and poly-fructose which are readily destroyed by acidic pretreatment, a two step process was used developed to depolymerized poly-fructose while maintaining its fermentability. The hydrolysate from before and after dilute acid processing was used in C. beijerinckii acetone and butanol fermentations with selected Agave species.more » Conclusions: Results have shown Agave s potential to be a source of fermentable sugars beyond the existing beverage species to now include species previously unfermentable by yeast, including cold tolerant lines. This development may stimulate development of Agave as a dedicated feedstock for biofuels in semi-arid regions throughout the globe.« less

Authors:
 [1];  [2];  [2];  [2];  [2]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.; White Cliff Biosystems, Rockwood, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Div.
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1185897
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
butanol; butyric acid; Clostridium beijerinckii; dedicated feedstock; fermentation; inulinase; ethanol; poly-fructose; semi-arid

Citation Formats

Mielenz, Jonathan R., Rodriguez, Jr, Miguel, Thompson, Olivia A, Yang, Xiaohan, and Yin, Hengfu. Development of Agave as a dedicated biomass source: production of biofuels from whole plants. United States: N. p., 2015. Web. doi:10.1186/s13068-015-0261-8.
Mielenz, Jonathan R., Rodriguez, Jr, Miguel, Thompson, Olivia A, Yang, Xiaohan, & Yin, Hengfu. Development of Agave as a dedicated biomass source: production of biofuels from whole plants. United States. doi:10.1186/s13068-015-0261-8.
Mielenz, Jonathan R., Rodriguez, Jr, Miguel, Thompson, Olivia A, Yang, Xiaohan, and Yin, Hengfu. Thu . "Development of Agave as a dedicated biomass source: production of biofuels from whole plants". United States. doi:10.1186/s13068-015-0261-8. https://www.osti.gov/servlets/purl/1185897.
@article{osti_1185897,
title = {Development of Agave as a dedicated biomass source: production of biofuels from whole plants},
author = {Mielenz, Jonathan R. and Rodriguez, Jr, Miguel and Thompson, Olivia A and Yang, Xiaohan and Yin, Hengfu},
abstractNote = {Background: Agave species can grow well in semi-arid marginal agricultural lands around the world. Selected Agave species are used largely for alcoholic beverage production in Mexico. There are expanding research efforts to use the plentiful residues (bagasse) for ethanol production as the beverage manufacturing process only uses the juice from the central core of mature plants. Here we investigate the potential of over a dozen Agave species, including three from cold semi-arid regions of the United States, to produce biofuels using the whole plant. Results: Ethanol was readily produced by Saccharomyces cerevisiae from hydrolysate of ten whole Agaves with the use of a proper blend of biomass degrading enzymes that overcomes toxicity of most of the species tested. Unlike yeast fermentations, Clostridium beijerinckii produced butanol plus acetone from nine species tested. Butyric acid, a precursor of butanol, was also present due to incomplete conversion during the screening process. Since Agave contains high levels of free and poly-fructose which are readily destroyed by acidic pretreatment, a two step process was used developed to depolymerized poly-fructose while maintaining its fermentability. The hydrolysate from before and after dilute acid processing was used in C. beijerinckii acetone and butanol fermentations with selected Agave species. Conclusions: Results have shown Agave s potential to be a source of fermentable sugars beyond the existing beverage species to now include species previously unfermentable by yeast, including cold tolerant lines. This development may stimulate development of Agave as a dedicated feedstock for biofuels in semi-arid regions throughout the globe.},
doi = {10.1186/s13068-015-0261-8},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 8,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2015},
month = {Thu Jan 01 00:00:00 EST 2015}
}

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Works referenced in this record:

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
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