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Title: Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments

Abstract

Cellulose nanocrystal materials have been labeled with modern Alexa Fluor dyes in a process that first links the dye to a cyanuric chloride molecule. Subsequent reaction with cellulose nanocrystals provides dyed solid microcrystalline cellulose material that can be used for bioimaging and suitable for deposition in films and spatially structured microenvironments. It is demonstrated with single molecular fluorescence microscopy that these films are subject to hydrolysis by cellulose enzymes.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1184284
Report Number(s):
PNNL-SA-88815
48240
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bioconjugate Chemistry, 26(3):593-601
Country of Publication:
United States
Language:
English
Subject:
Cellulose; Alexa Fluor; cellulose nocrystal; single molecule fluorescence microscopy; micromodel; microfluidic; cellulase; Environmental Molecular Sciences Laboratory

Citation Formats

Grate, Jay W., Mo, Kai-For, Shin, Yongsoon, Vasdekis, Andreas, Warner, Marvin G., Kelly, Ryan T., Orr, Galya, Hu, Dehong, Dehoff, Karl J., Brockman, Fred J., and Wilkins, Michael J.. Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments. United States: N. p., 2015. Web. doi:10.1021/acs.bioconjchem.5b00048.
Grate, Jay W., Mo, Kai-For, Shin, Yongsoon, Vasdekis, Andreas, Warner, Marvin G., Kelly, Ryan T., Orr, Galya, Hu, Dehong, Dehoff, Karl J., Brockman, Fred J., & Wilkins, Michael J.. Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments. United States. doi:10.1021/acs.bioconjchem.5b00048.
Grate, Jay W., Mo, Kai-For, Shin, Yongsoon, Vasdekis, Andreas, Warner, Marvin G., Kelly, Ryan T., Orr, Galya, Hu, Dehong, Dehoff, Karl J., Brockman, Fred J., and Wilkins, Michael J.. Wed . "Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments". United States. doi:10.1021/acs.bioconjchem.5b00048.
@article{osti_1184284,
title = {Alexa Fluor-labeled Fluorescent Cellulose Nanocrystals for Bioimaging Solid Cellulose in Spatially Structured Microenvironments},
author = {Grate, Jay W. and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G. and Kelly, Ryan T. and Orr, Galya and Hu, Dehong and Dehoff, Karl J. and Brockman, Fred J. and Wilkins, Michael J.},
abstractNote = {Cellulose nanocrystal materials have been labeled with modern Alexa Fluor dyes in a process that first links the dye to a cyanuric chloride molecule. Subsequent reaction with cellulose nanocrystals provides dyed solid microcrystalline cellulose material that can be used for bioimaging and suitable for deposition in films and spatially structured microenvironments. It is demonstrated with single molecular fluorescence microscopy that these films are subject to hydrolysis by cellulose enzymes.},
doi = {10.1021/acs.bioconjchem.5b00048},
journal = {Bioconjugate Chemistry, 26(3):593-601},
number = ,
volume = ,
place = {United States},
year = {Wed Mar 18 00:00:00 EDT 2015},
month = {Wed Mar 18 00:00:00 EDT 2015}
}