Method for chemical amplification based on fluid partitioning in an immiscible liquid

Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.

Method for chemical amplification based on fluid partitioning in an immiscible liquid

Patent · 02 June 2015

OSTI ID:1183336

Anderson, Brian L.; Colston, Bill W.; Elkin, Christopher J.

A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.

View Patent

Research Organization:: Lawrence Livermore National Security, LLC, Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: RE45,539

Application Number:: 13/436,693

OSTI ID:: 1183336

Country of Publication:: United States

Language:: English

References (45)

Principle and applications of digital PCR Pohl, Gudrun; Shih, Ie-Ming Expert Review of Molecular Diagnostics, Vol. 4, Issue 1 https://doi.org/10.1586/14737159.4.1.41	journal	January 2004
Mathematics of quantitative kinetic PCR and the application of standard curves Rutledge, R. G.; Cote, C. Nucleic Acids Research, Vol. 31, Issue 16, p. 93e-93 https://doi.org/10.1093/nar/gng093	journal	August 2003
Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms Clausell-Tormos, Jenifer; Lieber, Diana; Baret, Jean-Christophe Chemistry & Biology, Vol. 15, Issue 5, p. 427-437 https://doi.org/10.1016/j.chembiol.2008.04.004	journal	May 2008
Development of A Microchamber Array for Picoliter PCR Nagai, Hidenori; Murakami, Yuji; Morita, Yasutaka Analytical Chemistry, Vol. 73, Issue 5, p. 1043-1047 https://doi.org/10.1021/ac000648u	journal	March 2001
Amplification of complex gene libraries by emulsion PCR Williams, Richard; Peisajovich, Sergio G.; Miller, Oliver J. Nature Methods, Vol. 3, Issue 7, p. 545-550 https://doi.org/10.1038/nmeth896	journal	July 2006
Miniemulsion Droplets as Single Molecule Nanoreactors for Polymerase Chain Reaction Musyanovych, Anna; Mailänder, Volker; Landfester, Katharina Biomacromolecules, Vol. 6, Issue 4 https://doi.org/10.1021/bm050084+	journal	June 2005
Genome sequencing in microfabricated high-density picolitre reactors Margulies, Marcel; Egholm, Michael; Altman, William E. Nature, Vol. 437, Issue 7057, p. 376-380 https://doi.org/10.1038/nature03959	journal	July 2005
Droplet-based synthetic method using microflow focusing and droplet fusion Liu, Kan; Ding, Huijiang; Chen, Yong Microfluidics and Nanofluidics, Vol. 3, Issue 2, p. 239-243 https://doi.org/10.1007/s10404-006-0121-8	journal	September 2006
Microparticle Concentration and Separation by Traveling-Wave Dielectrophoresis (twDEP) for Digital Microfluidics Zhao, Yuejun; Yi, Ui-Chong; Cho, Sung Kwon Journal of Microelectromechanical Systems, Vol. 16, Issue 6, p. 1472-1481 https://doi.org/10.1109/JMEMS.2007.906763	journal	December 2007
Nanoliter scale PCR with TaqMan detection Kalinina, Olga; Lebedeva, Irina; Brown, James Nucleic Acids Research, Vol. 25, Issue 10, p. 1999-2004 https://doi.org/10.1093/nar/25.10.1999	journal	May 1997
Digital quantification using amplified single-molecule detection Jarvius, Jonas; Melin, Jonas; Göransson, Jenny Nature Methods, Vol. 3, Issue 9 https://doi.org/10.1038/nmeth916	journal	August 2006
Digital PCR for the molecular detection of fetal chromosomal aneuploidy Lo, Y. M. D.; Lun, F. M. F.; Chan, K. C. A. Proceedings of the National Academy of Sciences, Vol. 104, Issue 32, p. 13116-13121 https://doi.org/10.1073/pnas.0705765104	journal	July 2007
The Contribution of Combinatorial Chemistry to Lead Generation An Interim Analysis Adang, Anton; Hermkens, Pedro Current Medicinal Chemistry, Vol. 8, Issue 9 https://doi.org/10.2174/0929867013372517	journal	July 2001
Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions Higuchi, Russell; Fockler, Carita; Dollinger, Gavin Nature Biotechnology, Vol. 11, Issue 9 https://doi.org/10.1038/nbt0993-1026	journal	September 1993
Droplet Formation Utilizing Controllable Moving-Wall Structures for Double-Emulsion Applications Lin, Yen-Heng; Lee, Chun-Hong; Lee, Gwo-Bin Journal of Microelectromechanical Systems, Vol. 17, Issue 3, p. 573-581 https://doi.org/10.1109/JMEMS.2008.924273	journal	June 2008
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858 https://doi.org/10.1021/ac800048k	journal	March 2008
Chemical Amplification: Continuous-Flow PCR on a Chip Kopp, Martin U.; de Mello, Andrew J.; Manz, Andreas Science, Vol. 280, Issue 5366, p. 1046-1048 https://doi.org/10.1126/science.280.5366.1046	journal	May 1998
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets Kojima, Takaaki; Takei, Yoshiaki; Ohtsuka, Miharu Nucleic Acids Research, Vol. 33, Issue 17, p. e150-e150 https://doi.org/10.1093/nar/gni143	journal	September 2005
Droplet fusion by alternating current (AC) field electrocoalescence in microchannels Chabert, Max; Dorfman, Kevin D.; Viovy, Jean-Louis ELECTROPHORESIS, Vol. 26, Issue 19, p. 3706-3715 https://doi.org/10.1002/elps.200500109	journal	October 2005
Digital quantification of mutant DNA in cancer patients Diehl, Frank; Diaz, Luis A. Current Opinion in Oncology, Vol. 19, Issue 1, p. 36-42 https://doi.org/10.1097/CCO.0b013e328011a8e7	journal	January 2007
Single-molecule PCR using water-in-oil emulsion Nakano, Michihiko; Komatsu, Jun; Matsuura, Shun-ichi Journal of Biotechnology, Vol. 102, Issue 2, p. 117-124 https://doi.org/10.1016/S0168-1656(03)00023-3	journal	April 2003
Electric Control of Droplets in Microfluidic Devices Link, Darren R.; Grasland-Mongrain, Erwan; Duri, Agnes Angewandte Chemie International Edition, Vol. 45, Issue 16, p. 2556-2560 https://doi.org/10.1002/anie.200503540	journal	April 2006
Continuous flow separations in microfluidic devices Pamme, Nicole Lab on a Chip, Vol. 7, Issue 12, p. 1644-1659 https://doi.org/10.1039/b712784g	journal	January 2007
Droplet-Based Microfluidics for Emulsion and Solvent Evaporation Synthesis of Monodisperse Mesoporous Silica Microspheres Carroll, Nick J.; Rathod, Shailendra B.; Derbins, Erin Langmuir, Vol. 24, Issue 3, p. 658-661 https://doi.org/10.1021/la7032516	journal	February 2008
High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets Kiss, Margaret Macris; Ortoleva-Donnelly, Lori; Beer, N. Reginald Analytical Chemistry, Vol. 80, Issue 23, p. 8975-8981 https://doi.org/10.1021/ac801276c	journal	December 2008
Indirect micromanipulation of single molecules in water-in-oil emulsion Katsura, Shinji; Yamaguchi, Akihiro; Inami, Hiroyoshi ELECTROPHORESIS, Vol. 22, Issue 2, p. 289-293 https://doi.org/10.1002/1522-2683(200101)22:2<289::AID-ELPS289>3.0.CO;2-P	journal	January 2001
Megapixel digital PCR Heyries, Kevin A.; Tropini, Carolina; VanInsberghe, Michael Nature Methods, Vol. 8, Issue 8 https://doi.org/10.1038/nmeth.1640	journal	July 2011
Miniaturized flow-through PCR with different template types in a silicon chip thermocycler Schneegaß, Ivonne; Bräutigam, Reiner; Köhler, Johann Michael Lab Chip, Vol. 1, Issue 1 https://doi.org/10.1039/b103846j	journal	January 2001
Heat integration in micro-fluidic devices Zhelev, Toshko; Strelow, Olaf Computer Aided Chemical Engineering, Vol. 21, p. 1863-1868 https://doi.org/10.1016/S1570-7946(06)80319-6	book	January 2006
Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online Fidalgo, Luis M.; Whyte, Graeme; Ruotolo, Brandon T. Angewandte Chemie International Edition, Vol. 48, Issue 20, p. 3665-3668 https://doi.org/10.1002/anie.200806103	journal	May 2009
High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets Kumaresan, Palani; Yang, Chaoyong James; Cronier, Samantha A. Analytical Chemistry, Vol. 80, Issue 10, p. 3522-3529 https://doi.org/10.1021/ac800327d	journal	May 2008
On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K. Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475 https://doi.org/10.1021/ac701809w	journal	November 2007
Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications Rutledge, R. G. Nucleic Acids Research, Vol. 32, Issue 22, p. e178-e178 https://doi.org/10.1093/nar/gnh177	journal	January 2004
Using Three-Phase Flow of Immiscible Liquids To Prevent Coalescence of Droplets in Microfluidic Channels: Criteria To Identify the Third Liquid and Validation with Protein Crystallization Chen, Delai L.; Li, Liang; Reyes, Sebastian Langmuir, Vol. 23, Issue 4 https://doi.org/10.1021/la062152z	journal	February 2007
Controlling Nonspecific Protein Adsorption in a Plug-Based Microfluidic System by Controlling Interfacial Chemistry Using Fluorous-Phase Surfactants Roach, L. Spencer; Song, Helen; Ismagilov, Rustem F. Analytical Chemistry, Vol. 77, Issue 3 https://doi.org/10.1021/ac049061w	journal	February 2005
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Zhang, Chunsun; Xing, Da Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237 https://doi.org/10.1093/nar/gkm389	journal	June 2007
Thermocapillary valve for droplet production and sorting Baroud, Charles N.; Delville, Jean-Pierre; Gallaire, Francois Physical Review E, Vol. 75, Issue 4, Article No. 046302 https://doi.org/10.1103/PhysRevE.75.046302	journal	April 2007
Look before You Leap: Active Surveillance for Multidrug-Resistant Organisms Weinstein, Robert A.; Diekema, Daniel J.; Edmond, Michael B. Clinical Infectious Diseases, Vol. 44, Issue 8, p. 1101-1107 https://doi.org/10.1086/512820	journal	April 2007
Klenow fragment and DNA polymerase α-primase fromserva calf thymus in water-in-oil microemulsions Anarbaev, Rashid O.; Elepov, Ilja B.; Lavrik, Olga I. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1384, Issue 2 https://doi.org/10.1016/S0167-4838(98)00025-9	journal	May 1998
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations Dressman, D.; Yan, H.; Traverso, G. Proceedings of the National Academy of Sciences, Vol. 100, Issue 15, p. 8817-8822 https://doi.org/10.1073/pnas.1133470100	journal	July 2003
A microfluidic droplet generator based on a piezoelectric actuator Bransky, Avishay; Korin, Natanel; Khoury, Maria Lab Chip, Vol. 9, Issue 4, p. 516-520 https://doi.org/10.1039/b814810d	journal	January 2009
Overview: methods and applications for droplet compartmentalization of biology Leamon, John H.; Link, Darren R.; Egholm, Michael Nature Methods, Vol. 3, Issue 7 https://doi.org/10.1038/nmeth0706-541	journal	July 2006
Behavioral Modeling and Performance Evaluation of Microelectrofluidics-Based PCR Systems Using SystemC Zhang, Tianhao; Chakrabarty, K.; Fair, R. B. IEEE Transactions on Computer-Aided Design of Integrated Circuits and Systems, Vol. 23, Issue 6, p. 843-858 https://doi.org/10.1109/TCAD.2004.828115	journal	June 2004
Digital PCR Vogelstein, B.; Kinzler, K. W. Proceedings of the National Academy of Sciences, Vol. 96, Issue 16 https://doi.org/10.1073/pnas.96.16.9236	journal	August 1999
All-terrain droplet actuation Abdelgawad, Mohamed; Freire, Sergio L. S.; Yang, Hao Lab on a Chip, Vol. 8, Issue 5 https://doi.org/10.1039/b801516c	journal	January 2008

Similar Records

Method for chemical amplification based on fluid partitioning in an immiscible liquid

Patent · 2017 · OSTI ID:1345204

Chemical amplification based on fluid partitioning in an immiscible liquid

Patent · 2010 · OSTI ID:1176714

Apparatus for chemical amplification based on fluid partitioning in an immiscible liquid

Patent · 2012 · OSTI ID:1176720

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Method for chemical amplification based on fluid partitioning in an immiscible liquid

Citation Formats

References (45)

Similar Records

Related Subjects