Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

Journal Article · Fri May 01 00:00:00 EDT 2015 · Biomicrofluidics, 9(3):Article No. 031101

DOI:https://doi.org/10.1063/1.4919807· OSTI ID:1182885

Hua, Xin; Marshall, Matthew J.; Xiong, Yijia; Ma, Xiang; Zhou, Yufan; Tucker, Abigail E.; Zhu, Zihua; Liu, Songqin; Yu, Xiao-Ying

A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface) was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. 2D images were reconstructed to report the first 3D images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis (PCA) was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1182885
• Report Number(s): PNNL-SA-105631; 47299; 47484; 44631; 36601; 48143; 42330; KP1601010
• Journal Information: Biomicrofluidics, 9(3):Article No. 031101, Journal Name: Biomicrofluidics, 9(3):Article No. 031101
• Country of Publication: United States
• Language: English

Related Subjects

in situ imaging
microfluidic
vacuum
biofilm
3D image reconstruction
image principal component analysis Early Career Research Award
Environmental Molecular Sciences Laboratory