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Title: Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

Abstract

A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface) was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. 2D images were reconstructed to report the first 3D images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis (PCA) was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1182885
Report Number(s):
PNNL-SA-105631
47299; 47484; 44631; 36601; 48143; 42330; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Biomicrofluidics, 9(3):Article No. 031101
Additional Journal Information:
Journal Name: Biomicrofluidics, 9(3):Article No. 031101
Country of Publication:
United States
Language:
English
Subject:
in situ imaging, microfluidic, vacuum, biofilm, 3D image reconstruction, image principal component analysis Early Career Research Award; Environmental Molecular Sciences Laboratory

Citation Formats

Hua, Xin, Marshall, Matthew J., Xiong, Yijia, Ma, Xiang, Zhou, Yufan, Tucker, Abigail E., Zhu, Zihua, Liu, Songqin, and Yu, Xiao-Ying. Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry. United States: N. p., 2015. Web. doi:10.1063/1.4919807.
Hua, Xin, Marshall, Matthew J., Xiong, Yijia, Ma, Xiang, Zhou, Yufan, Tucker, Abigail E., Zhu, Zihua, Liu, Songqin, & Yu, Xiao-Ying. Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry. United States. doi:10.1063/1.4919807.
Hua, Xin, Marshall, Matthew J., Xiong, Yijia, Ma, Xiang, Zhou, Yufan, Tucker, Abigail E., Zhu, Zihua, Liu, Songqin, and Yu, Xiao-Ying. Fri . "Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry". United States. doi:10.1063/1.4919807.
@article{osti_1182885,
title = {Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry},
author = {Hua, Xin and Marshall, Matthew J. and Xiong, Yijia and Ma, Xiang and Zhou, Yufan and Tucker, Abigail E. and Zhu, Zihua and Liu, Songqin and Yu, Xiao-Ying},
abstractNote = {A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface) was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. 2D images were reconstructed to report the first 3D images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis (PCA) was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.},
doi = {10.1063/1.4919807},
journal = {Biomicrofluidics, 9(3):Article No. 031101},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {5}
}