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Title: Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis

Abstract

Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the key biological processes involved. We applied a combination of metabolomic and lipidomic profiling approaches as well as microscopic techniques to identify and characterize the key pathways involved in de novo lipid accumulation from glucose in batch cultured, wild-type Y. lipolytica. We found that lipids accumulated rapidly and peaked at 48 hours during the five day experiment, concurrent with a shift in amino acid metabolism. We also report that Y. lipolytica secretes disaccharides early in batch culture and reabsorbs them when extracellular glucose is depleted. Exhaustion of extracellular sugars coincided with thickening of the cell wall, suggesting that genes involved in cell wall biogenesis may be a useful target for improving the efficiency of lipid producing yeast strains.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1179505
Report Number(s):
PNNL-SA-105704
48167; 47736; KP1601010
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS One, 10(4):e0123188
Additional Journal Information:
Journal Volume: 10; Journal Issue: 4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; yarrowia plos paper; Environmental Molecular Sciences Laboratory

Citation Formats

Pomraning, Kyle R., Wei, Siwei, Karagiosis, Sue A., Kim, Young-Mo, Dohnalkova, Alice, Arey, Bruce W., Bredeweg, Erin L., Orr, Galya, Metz, Thomas O., and Baker, Scott E. Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis. United States: N. p., 2015. Web. doi:10.1371/journal.pone.0123188.
Pomraning, Kyle R., Wei, Siwei, Karagiosis, Sue A., Kim, Young-Mo, Dohnalkova, Alice, Arey, Bruce W., Bredeweg, Erin L., Orr, Galya, Metz, Thomas O., & Baker, Scott E. Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis. United States. doi:10.1371/journal.pone.0123188.
Pomraning, Kyle R., Wei, Siwei, Karagiosis, Sue A., Kim, Young-Mo, Dohnalkova, Alice, Arey, Bruce W., Bredeweg, Erin L., Orr, Galya, Metz, Thomas O., and Baker, Scott E. Thu . "Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis". United States. doi:10.1371/journal.pone.0123188. https://www.osti.gov/servlets/purl/1179505.
@article{osti_1179505,
title = {Comprehensive metabolomic, lipidomic and microscopic profiling of Yarrowia lipolytica during lipid accumulation identifies targets for increased lipogenesis},
author = {Pomraning, Kyle R. and Wei, Siwei and Karagiosis, Sue A. and Kim, Young-Mo and Dohnalkova, Alice and Arey, Bruce W. and Bredeweg, Erin L. and Orr, Galya and Metz, Thomas O. and Baker, Scott E.},
abstractNote = {Yarrowia lipolytica is an oleaginous ascomycete yeast that accumulates large amounts of lipids and has potential as a biofuel producing organism. Despite a growing scientific literature focused on lipid production by Y. lipolytica, there remain significant knowledge gaps regarding the key biological processes involved. We applied a combination of metabolomic and lipidomic profiling approaches as well as microscopic techniques to identify and characterize the key pathways involved in de novo lipid accumulation from glucose in batch cultured, wild-type Y. lipolytica. We found that lipids accumulated rapidly and peaked at 48 hours during the five day experiment, concurrent with a shift in amino acid metabolism. We also report that Y. lipolytica secretes disaccharides early in batch culture and reabsorbs them when extracellular glucose is depleted. Exhaustion of extracellular sugars coincided with thickening of the cell wall, suggesting that genes involved in cell wall biogenesis may be a useful target for improving the efficiency of lipid producing yeast strains.},
doi = {10.1371/journal.pone.0123188},
journal = {PLoS One, 10(4):e0123188},
number = 4,
volume = 10,
place = {United States},
year = {2015},
month = {4}
}

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Cited by: 19 works
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