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Title: Hydrogel nanoparticle based immunoassay

Abstract

An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.

Inventors:
; ; ;
Publication Date:
Research Org.:
George Mason Research Foundation, Inc., Fairfax, VA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1178255
Patent Number(s):
9,012,240
Application Number:
13/061,507
Assignee:
George Mason Research Foundation, Inc. (Fairfax, VA) NNSASC
DOE Contract Number:  
FC52-04NA25455
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Aug 26
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, and Espina, Virginia. Hydrogel nanoparticle based immunoassay. United States: N. p., 2015. Web.
Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, & Espina, Virginia. Hydrogel nanoparticle based immunoassay. United States.
Liotta, Lance A, Luchini, Alessandra, Petricoin, Emanuel F, and Espina, Virginia. Tue . "Hydrogel nanoparticle based immunoassay". United States. doi:. https://www.osti.gov/servlets/purl/1178255.
@article{osti_1178255,
title = {Hydrogel nanoparticle based immunoassay},
author = {Liotta, Lance A and Luchini, Alessandra and Petricoin, Emanuel F and Espina, Virginia},
abstractNote = {An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 21 00:00:00 EDT 2015},
month = {Tue Apr 21 00:00:00 EDT 2015}
}

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Works referenced in this record:

Ligand-Functionalized Core/Shell Microgels with Permselective Shells
journal, December 2004

  • Nayak, Satish; Lyon, L. Andrew
  • Angewandte Chemie International Edition, Vol. 43, Issue 48, p. 6706-6709
  • DOI: 10.1002/anie.200461090