A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography
Abstract
Racemic protein crystallography was used to determine the X-ray structure of the predicted Mycobacterium tuberculosis protein Rv1738, which had been completely recalcitrant to crystallization in its natural L-form. Native chemical ligation was used to synthesize both L-protein and D-protein enantiomers of Rv1738. Crystallization of the racemic {D-protein + L-protein} mixture was immediately successful. The resulting crystals diffracted to high resolution and also enabled facile structure determination because of the quantized phases of the data from centrosymmetric crystals. The X-ray structure of Rv1738 revealed striking similarity with bacterial hibernation factors, despite minimal sequence similarity. As a result, we predict that Rv1738, which is highly up-regulated in conditions that mimic the onset of persistence, helps trigger dormancy by association with the bacterial ribosome.
- Authors:
-
- Univ. of Auckland, Auckland (New Zealand)
- Univ. of Chicago, Chicago, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1178127
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 112; Journal Issue: 14; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; racemic protein crystallography; Mycobacterium tuberculosis; ribosome binding; dormancy; protein structure
Citation Formats
Bunker, Richard D., Mandal, Kalyaneswar, Bashiri, Ghader, Chaston, Jessica J., Pentelute, Bradley L., Lott, J. Shaun, Kent, Stephen B. H., and Baker, Edward N. A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography. United States: N. p., 2015.
Web. doi:10.1073/pnas.1422387112.
Bunker, Richard D., Mandal, Kalyaneswar, Bashiri, Ghader, Chaston, Jessica J., Pentelute, Bradley L., Lott, J. Shaun, Kent, Stephen B. H., & Baker, Edward N. A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography. United States. https://doi.org/10.1073/pnas.1422387112
Bunker, Richard D., Mandal, Kalyaneswar, Bashiri, Ghader, Chaston, Jessica J., Pentelute, Bradley L., Lott, J. Shaun, Kent, Stephen B. H., and Baker, Edward N. 2015.
"A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography". United States. https://doi.org/10.1073/pnas.1422387112. https://www.osti.gov/servlets/purl/1178127.
@article{osti_1178127,
title = {A functional role of Rv1738 in Mycobacterium tuberculosis persistence suggested by racemic protein crystallography},
author = {Bunker, Richard D. and Mandal, Kalyaneswar and Bashiri, Ghader and Chaston, Jessica J. and Pentelute, Bradley L. and Lott, J. Shaun and Kent, Stephen B. H. and Baker, Edward N.},
abstractNote = {Racemic protein crystallography was used to determine the X-ray structure of the predicted Mycobacterium tuberculosis protein Rv1738, which had been completely recalcitrant to crystallization in its natural L-form. Native chemical ligation was used to synthesize both L-protein and D-protein enantiomers of Rv1738. Crystallization of the racemic {D-protein + L-protein} mixture was immediately successful. The resulting crystals diffracted to high resolution and also enabled facile structure determination because of the quantized phases of the data from centrosymmetric crystals. The X-ray structure of Rv1738 revealed striking similarity with bacterial hibernation factors, despite minimal sequence similarity. As a result, we predict that Rv1738, which is highly up-regulated in conditions that mimic the onset of persistence, helps trigger dormancy by association with the bacterial ribosome.},
doi = {10.1073/pnas.1422387112},
url = {https://www.osti.gov/biblio/1178127},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 14,
volume = 112,
place = {United States},
year = {Tue Apr 07 00:00:00 EDT 2015},
month = {Tue Apr 07 00:00:00 EDT 2015}
}
Web of Science
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