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Title: Devices, systems, and methods for microscale isoelectric fractionation

Abstract

Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.

Inventors:
; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1178015
Patent Number(s):
9,005,417
Application Number:
12/243,817
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Oct 01
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sommer, Gregory J, Hatch, Anson V, Wang, Ying-Chih, and Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States: N. p., 2015. Web.
Sommer, Gregory J, Hatch, Anson V, Wang, Ying-Chih, & Singh, Anup K. Devices, systems, and methods for microscale isoelectric fractionation. United States.
Sommer, Gregory J, Hatch, Anson V, Wang, Ying-Chih, and Singh, Anup K. Tue . "Devices, systems, and methods for microscale isoelectric fractionation". United States. doi:. https://www.osti.gov/servlets/purl/1178015.
@article{osti_1178015,
title = {Devices, systems, and methods for microscale isoelectric fractionation},
author = {Sommer, Gregory J and Hatch, Anson V and Wang, Ying-Chih and Singh, Anup K},
abstractNote = {Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 14 00:00:00 EDT 2015},
month = {Tue Apr 14 00:00:00 EDT 2015}
}

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