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Buoyant triacylglycerol-filled green algae and methods therefor

Patent ·
OSTI ID:1178007
;

Cultures of Chlamydomonas are disclosed comprising greater than 340 mg/l triacylglycerols (TAG). The cultures can include buoyant Chlamydomonas. Methods of forming the cultures are also disclosed. In some embodiments, these methods comprise providing Chlamydomonas growing in log phase in a first culture medium comprising a nitrogen source and acetate, replacing the first culture medium with a second medium comprising acetate but no nitrogen source, and subsequently supplementing the second medium with additional acetate. In some embodiments, a culture can comprise at least 1,300 mg/l triacyglycerols. In some embodiments, cultures can be used to produce a biofuel such as biodiesel.

Research Organization:
Washington University, Saint Louis, MO (United States)
Sponsoring Organization:
USDOE
Assignee:
Washington University (Saint Louis, MO)
Patent Number(s):
9,005,955
Application Number:
13/662,549
OSTI ID:
1178007
Country of Publication:
United States
Language:
English

References (19)

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