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Method of identifying hairpin DNA probes by partial fold analysis

Patent ·
OSTI ID:1176186
;
Methods of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.
Research Organization:
University Of Rochester, Rochester, NY (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-02ER63410
Assignee:
University Of Rochester (Rochester, NY)
Patent Number(s):
7,442,510
Application Number:
11/553,904
OSTI ID:
1176186
Country of Publication:
United States
Language:
English

References (7)

Effect of Secondary Structure on the Thermodynamics and Kinetics of PNA Hybridization to DNA Hairpins journal November 2001
Hybridization of single-stranded DNA targets to immobilized complementary DNA probes: comparison of hairpin versus linear capture probes journal February 2001
Thermodynamic basis of the enhanced specificity of structured DNA probes journal May 1999
Stem-loop oligonucleotides: a robust tool for molecular biology and biotechnology journal June 2002
Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon journal January 2000
Single-mismatch detection using gold-quenched fluorescent oligonucleotides journal April 2001
Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces:  Prototypical “Molecular Beacon” Biosensors journal April 2003

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