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Title: Method of rapid production of hybridomas expressing monoclonal antibodies on the cell surface

Patent ·
OSTI ID:1176025

The present invention relates to genetically altered hybridomas, myelomas and B cells. The invention also relates to utilizing genetically altered hybridomas, myelomas and B cells in methods of making monoclonal antibodies. The present invention also provides populations of hybridomas and B cells that can be utilized to make a monoclonal antibody of interest.

Research Organization:
University of Georgia, Athens, GA (Unites States); Abeome Corporation, Athens, GA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG07-96ER20257
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA) ; Abeome Corporation (Athens, GA)
Patent Number(s):
7,148,040
Application Number:
10/079,130
OSTI ID:
1176025
Resource Relation:
Patent File Date: 2002 Feb 20
Country of Publication:
United States
Language:
English

References (77)

Molecular cloning of the Ig-? subunit of the human B-cell antigen receptor complex journal July 1992
The membrane IgM-associated proteins MB-1 and Ig-beta are sufficient to promote surface expression of a partially functional B-cell antigen receptor in a nonlymphoid cell line. journal April 1992
Transcriptional and posttranscriptional control of immunoglobulin mRNA production during Blymphocyte development journal January 1986
B Cell Development Is Arrested at the Immature B Cell Stage in Mice Carrying a Mutation in the Cytoplasmic Domain of Immunoglobulin β journal December 2000
Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation journal February 1999
Clinical trials of antibody therapy journal August 2000
Treatment of B-Cell Lymphoma with Monoclonal Anti-Idiotype Antibody journal March 1982
Signal Transduction by the Antigen Receptors of B and T Lymphocytes book January 1995
The metabolism of a poly(A) minus mRNA fraction in HeLa cells journal September 1974
Application of flow cytometric measurement of surface IgG in kinetic analysis of monoclonal antibody synthesis and secretion by murine hybridoma cells journal July 1989
Optimizing antibody production in batch hybridoma cell culture journal May 1995
The B-cell antigen receptor complex journal June 1991
A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization journal December 1998
B lymphocyte lineage-restricted expression of mb-1, a gene with CD3-like structural properties. journal November 1988
Aberrant B Cell Development and Immune Response in Mice with a Compromised BCR Complex journal June 1996
Igα: B all that you can B journal October 1999
Cell-specific expression of secreted versus membrane forms of immunoglobulin gamma 2b mRNA involves selective use of alternate polyadenylation sites. journal October 1985
B Cell Progenitors Are Arrested in Maturation but Have Intact VDJ Recombination in the Absence of Ig-α and Ig-β journal July 2002
An unsolved problem of the clonal selection theory and the model of an oligomeric B-cell antigen receptor journal August 2000
Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion journal July 1976
Ig-α Cytoplasmic Truncation Renders Immature B Cells More Sensitive to Antigen Contact journal November 1999
Antigen Receptors on B Lymphocytes journal April 1992
Relative position and strengths of poly(A) sites as well as transcription termination are critical to membrane versus secreted mu-chain expression during B-cell development. journal July 1987
Immunoglobulin gene expression in transformed lymphoid cells. journal February 1983
Changes in abundance of IgG 2a mRNA in the nucleus and cytoplasm of a murine B-lymphoma before and after fusion to a myeloma cell journal May 1996
CH gene rearrangements in IgM-bearing B cells and in the normal splenic DNA component of hybridomas making different isotypes of antibody journal November 1980
Identification of the genes encoding the IgM-α and Ig-β components of the IgM antigen receptor complex by amino-terminal sequencing journal December 1990
Increased half-life of μ immunoglobulin mRNA during mouse B cell development increases its abundancy journal August 1990
Alternative poly(A) site selection in complex transcription units: means to an end? journal July 1997
Differential mRNA stabilities affect mRNA levels in mutant mouse myeloma cells journal January 1991
The internalization of the IgG2a antigen receptor does not require the association with Ig-α and Ig-β but the activation of protein tyrosine kinases does journal March 1994
Analysis of receptor signaling pathways by mass spectrometry: Identification of Vav-2 as a substrate of the epidermal and platelet-derived growth factor receptors journal January 2000
Monoclonal antibodies to beta-adrenergic receptors: use in purification and molecular characterization of beta receptors. journal December 1980
Scaffolds for engineering novel binding sites in proteins journal August 1997
Human Keratinocytes That Express hTERT and Also Bypass a p16INK4a-Enforced Mechanism That Limits Life Span Become Immortal yet Retain Normal Growth and Differentiation Characteristics journal February 2000
50 million years of chordate evolution: Seeking the origins of adaptive immunity journal June 2000
Contribution of a single heavy chain residue to specificity of an anti-digoxin monoclonal antibody: Molecular analysis of altered antibody specificity journal May 1994
Alternative splicing of CD79a (Ig-αmb-1) and CD79b (Ig-βB29) RNA transcripts in human B cells journal June 1995
Dual role of the tyrosine activation motif of the Ig-alpha protein during signal transduction via the B cell antigen receptor. journal January 1994
Sequence and polyadenylation site determination of the murine immunoglobulin γ2a membrane 3' untranslated region journal September 1989
Sequences near the 3' secretion-specific polyadenylation site influence levels of secretion-specific and membrane-specific IgG2b mRNA in myeloma cells. journal May 1986
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
Printing Proteins as Microarrays for High-Throughput Function Determination journal September 2000
Molecular components of the B-cell antigen receptor complex of the IgM class journal February 1990
Differential signaling through the Ig-α and Ig-β components of the B cell antigen receptor journal April 1993
Rapid Isolation of Monoclonal Antibodies. Monitoring Enzymes in the Phytochelatin Synthesis Pathway journal November 2001
Regulation of nuclear poly(A) addition controls the expression of immunoglobulin M secretory mRNA journal November 2001
Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells journal November 1998
The cytoplasmic domains of immunoglobulin (Ig) alpha and Ig beta can independently induce the precursor B cell transition and allelic exclusion. journal November 1995
Use of Monoclonal Antibodies for the Diagnosis and Treatment of Bladder Cancer journal June 1999
Rapid Hybridoma Screening Method for the Identification of Monoclonal Antibodies to Low-Abundance Cytoplasmic Proteins journal November 1998
Aberrant Trafficking of the B Cell Receptor Ig-αβ Subunit in a B Lymphoma Cell Line journal August 2000
Transcriptional regulation of the mu-delta heavy chain locus in normal murine B lymphocytes. journal August 1984
Genomic Instability and Telomerase Activity in Human Bronchial Epithelial Cells during Immortalization by Human Papillomavirus-16 E6 and E7 Genes journal August 1997
The hybridoma revolution: an offshoot of basic research journal October 1999
Monoclonal antibodies to human estrogen receptor. journal September 1980
With the benefit of hindsight journal August 2000
Stability of the B cell antigen receptor complex journal April 2000
Immortalization of human embryonic fibroblasts by overexpression of c-myc and simian virus 40 large T antigen journal December 2001
The Dictyostelium Developmental cDNA Project: Generation and Analysis of Expressed Sequence Tags from the First-Finger Stage of Development journal January 1998
Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter. journal April 1979
Genomics, gene expression and DNA arrays journal June 2000
Renaissance of cancer therapeutic antibodies journal June 2003
Cyclin D1 overexpression and p53 inactivation immortalize primary oral keratinocytes by a telomerase-independent mechanism journal September 2001
Proteomics journal April 1998
Structure and Function of the B-Cell Antigen Receptor book January 1994
B-lineage regulated polyadenylation occurs on weak poly(A) sites regardless of sequence composition at the cleavage and downstream regions journal December 1996
Loss of Antibody Productivity During Long-Term Cultivation of a Hybridoma Cell Line in Low Serum and Serum-Free Media journal April 1990
The late pollen-specific actins in angiosperms journal June 1999
IgG production in hybridoma batch culture: kinetics of IgG mRNA, cytoplasmic-, secreted- and membrane-bound antibody levels journal October 1991
Regulation of poly(A) site use during mouse B-cell development involves a change in the binding of a general polyadenylation factor in a B-cell stage-specific manner. journal November 1995
The 3′-Untranslated Region of Membrane Exon 2 from the γ2a Immunoglobulin Gene Contributes to Efficient Transcription Termination journal May 1995
Genetic diversity of the human immunoglobulin heavy chain VH region journal December 2002
Proteomics to study genes and genomes journal June 2000
Reconstitution of B Cell Antigen Receptor-induced Signaling Events in a Nonlymphoid Cell Line by Expressing the Syk Protein-tyrosine Kinase journal March 1996
Antibody engineering via genetic engineering of the mouse: XenoMouse strains are a vehicle for the facile generation of therapeutic human monoclonal antibodies journal December 1999
Molecular components of the B cell antigen receptor complex of class IgD differ partly from those of IgM. journal February 1990