Methods and compositions for simultaneous saccharification and fermentation

Ingram, Lonnie O'Neal; Zhou, Shengde

Title: Methods and compositions for simultaneous saccharification and fermentation

Patent · Tue Apr 11 00:00:00 EDT 2006

OSTI ID:1175699

Ingram, Lonnie O'Neal; Zhou, Shengde

The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-96ER20222

Assignee:: University of Florida Research Foundation, Inc. (Gainesville, FL)

Patent Number(s):: 7,026,152

Application Number:: 09/885,297

OSTI ID:: 1175699

Country of Publication:: United States

Language:: English

References (51)

Ethanol production from starch by recombinantEscherichia coli containing integrated genes for ethanol production and plasmid genes for saccharification Guimaraes, Walter V.; Ohta, Kazuyoshi; Burchhardt, Gerhard Biotechnology Letters, Vol. 14, Issue 5 https://doi.org/10.1007/BF01021257	journal	May 1992
Degradation of microcrystalline cellulose: Synergism between different endoglucanases ofCellulomonas sp. ATCC 21399 Poulsen, O. M.; Petersen, L. W. Biotechnology and Bioengineering, Vol. 39, Issue 1 https://doi.org/10.1002/bit.260390118	journal	January 1992
Expression of Different Levels of Ethanologenic Enzymes from Zymomonas mobilis in Recombinant Strains of Escherichia coli† Ingram, L. O.; Conway, T. Applied and Environmental Microbiology, Vol. 54, Issue 2 https://doi.org/10.1128/AEM.54.2.397-404.1988	journal	January 1988
Conversion of Mixed Waste Office Paper to Ethanol by Genetically Engineered Klebsiella oxytoca Strain P2 Brooks, Todd A.; Ingram, L. O. Biotechnology Progress, Vol. 11, Issue 6 https://doi.org/10.1021/bp00036a003	journal	November 1995
An interlaboratory comparison of the performance of ethanol-producing micro-organisms in a xylose-rich acid hydroysate Hahn-H�gerdal, B.; Jeppsson, H.; Olsson, L. Applied Microbiology and Biotechnology, Vol. 41, Issue 1 https://doi.org/10.1007/BF00166083	journal	March 1994
Complementation of deletion mutations in a cloned functional cluster of Erwinia chrysanthemi out genes with Erwinia carotovora out homologues reveals OutC and OutD as candidate gatekeepers of species-specific secretion of proteins via the type II pathway Lindeberg, Magdalen; Salmond, George P. C.; Collmer, Alan Molecular Microbiology, Vol. 20, Issue 1 https://doi.org/10.1111/j.1365-2958.1996.tb02499.x	journal	April 1996
Fuel Ethanol from Cellulosic Biomass Lynd, L. R.; Cushman, J. H.; Nichols, R. J. Science, Vol. 251, Issue 4999 https://doi.org/10.1126/science.251.4999.1318	journal	March 1991
Parametric studies of ethanol production form xylose and other sugars by recombinantEscherichia coli Beall, David S.; Ohta, K.; Ingram, L. O. Biotechnology and Bioengineering, Vol. 38, Issue 3 https://doi.org/10.1002/bit.260380311	journal	July 1991
Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Wood, B. E.; Ingram, L. O. Applied and Environmental Microbiology, Vol. 58, Issue 7 https://doi.org/10.1128/AEM.58.7.2103-2110.1992	journal	January 1992
Purification and characterization of endoglucanase C from Clostridium cellulolyticum. Catalytic comparison with endoglucanase A Fierobe, Henri-Pierre; Bagnara-Tardif, Chantal; Gaudin, Christian European Journal of Biochemistry, Vol. 217, Issue 2 https://doi.org/10.1111/j.1432-1033.1993.tb18277.x	journal	October 1993
Intramolecular synergism in an engineered exo-endo-1,4-β-glucanase fusion protein: Intramolecular synergism in an engineered cellulase Riedel, Kathrin; Bronnenmeier, Karin Molecular Microbiology, Vol. 28, Issue 4 https://doi.org/10.1046/j.1365-2958.1998.00834.x	journal	May 1998
Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. He, S. Y.; Lindeberg, M.; Chatterjee, A. K. Proceedings of the National Academy of Sciences, Vol. 88, Issue 3 https://doi.org/10.1073/pnas.88.3.1079	journal	February 1991
Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus Barbosa, Maria de F. S.; Ingram, L. O. Current Microbiology, Vol. 28, Issue 5 https://doi.org/10.1007/BF01573206	journal	May 1994
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans Figurski, D. H.; Helinski, D. R. Proceedings of the National Academy of Sciences, Vol. 76, Issue 4 https://doi.org/10.1073/pnas.76.4.1648	journal	April 1979
Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) fromErwinia chrysanthemi † Zhou, Shengde; Ingram, Lonnie O. Journal of Bacteriology, Vol. 182, Issue 20 https://doi.org/10.1128/JB.182.20.5676-5682.2000	journal	January 2000
Saccharification and fermentation of Sugar Cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway Doran, Joy B.; Aldrich, H. C.; Ingram, L. O. Biotechnology and Bioengineering, Vol. 44, Issue 2, p. 240-247 https://doi.org/10.1002/bit.260440213	journal	June 1994
Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2 Zhou, S.; Ingram, L. O. Journal of Industrial Microbiology and Biotechnology, Vol. 22, Issue 6 https://doi.org/10.1038/sj.jim.2900666	journal	June 1999
Enhancement of Expression and Apparent Secretion ofErwinia chrysanthemi Endoglucanase (Encoded bycelZ) in Escherichia coli B Zhou, Shengde; Yomano, Lorraine P.; Saleh, Alif Z. Applied and Environmental Microbiology, Vol. 65, Issue 6 https://doi.org/10.1128/AEM.65.6.2439-2445.1999	journal	June 1999
Characterization of transposon insertion out- mutants of Erwinia carotovora subsp. carotovora defective in enzyme export and of a DNA segment that complements out mutations in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Erwinia chrysanthemi. Murata, H.; Fons, M.; Chatterjee, A. Journal of Bacteriology, Vol. 172, Issue 6 https://doi.org/10.1128/JB.172.6.2970-2978.1990	journal	January 1990
Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes Doran, Joy B.; Ingram, L. O. Biotechnology Progress, Vol. 9, Issue 5 https://doi.org/10.1021/bp00023a013	journal	September 1993
Enteric Bacterial Catalysts for Fuel Ethanol Production Ingram, L. O.; Aldrich, H. C.; Borges, A. C. C. Biotechnology Progress, Vol. 15, Issue 5 https://doi.org/10.1021/bp9901062	journal	October 1999
Chromosomal Integration of Heterologous DNA in Escherichia coli with Precise Removal of Markers and Replicons Used during Construction Martinez-Morales, F.; Borges, A. C.; Martinez, A. Journal of Bacteriology, Vol. 181, Issue 22 https://doi.org/10.1128/JB.181.22.7143-7148.1999	journal	November 1999
Extracellular Enzymes and Pathogenesis of Soft-Rot Erwinia Barras, F.; van Gijsegem, F.; Chatterjee, A. K. Annual Review of Phytopathology, Vol. 32, Issue 1 https://doi.org/10.1146/annurev.py.32.090194.001221	journal	September 1994
Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes Liebl, W.; Ruile, P.; Bronnenmeier, K. Microbiology, Vol. 142, Issue 9 https://doi.org/10.1099/00221287-142-9-2533	journal	September 1996
Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Lai, X.; Davis, F. C.; Hespell, R. B. Applied and environmental microbiology, Vol. 63, Issue 2 https://doi.org/10.1128/AEM.63.2.355-363.1997	journal	January 1997
Fermentation of galacturonic acid and other sugars in orange peel hydrolysates by the ethanologenic strain of Escherichia coli Grohmann, K.; Baldwin, E. A.; Buslig, B. S. Biotechnology Letters, Vol. 16, Issue 3 https://doi.org/10.1007/BF00134626	journal	March 1994
Gene Integration and Expression and Extracellular Secretion of Erwinia chrysanthemi Endoglucanase CelY (celY) and CelZ (celZ) in Ethanologenic Klebsiella oxytoca P2 Zhou, S.; Davis, F. C.; Ingram, L. O. Applied and Environmental Microbiology, Vol. 67, Issue 1 https://doi.org/10.1128/AEM.67.1.6-14.2001	journal	January 2001
Ethanol production from AFEX pretreated corn fiber by recombinant bacteria Moniruzzaman, Mohammed; Dien, Bruce S.; Ferrer, Betzabe Biotechnology Letters, Vol. 18, Issue 8 https://doi.org/10.1007/BF00154635	journal	August 1996
Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineeredEscherichia coli strain KO11 Asghari, A.; Bothast, R. J.; Doran, J. B. Journal of Industrial Microbiology, Vol. 16, Issue 1 https://doi.org/10.1007/BF01569920	journal	January 1996
Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production Yomano, L. P.; York, S. W.; Ingram, L. O. Journal of Industrial Microbiology and Biotechnology, Vol. 20, Issue 2 https://doi.org/10.1038/sj.jim.2900496	journal	February 1998
Synergism in cellulase systems Woodward, Jonathan Bioresource Technology, Vol. 36, Issue 1 https://doi.org/10.1016/0960-8524(91)90100-X	journal	January 1991
Isolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi. Homology between cel genes of two strains of Erwinia chrysanthemi Boyer, Mari-HÃ©lÃ¨ne; Cami, Brigitte; Kotoujansky, Alain FEMS Microbiology Letters, Vol. 41, Issue 3 https://doi.org/10.1111/j.1574-6968.1987.tb02225.x	journal	May 1987
Fermentation of sweet whey by ethanologenicEscherichia coli Guimaraes, Walter V.; Dudey, Gregory L.; Ingram, L. O. Biotechnology and Bioengineering, Vol. 40, Issue 1 https://doi.org/10.1002/bit.260400107	journal	June 1992
Methods for measuring cellulase activities Wood, Thomas M.; Bhat, K. Mahalingeshwara Methods in Enzymology, Vol. 160, p. 87-112 https://doi.org/10.1016/0076-6879(88)60109-1	journal	January 1988
Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer Guiseppi, Annick; Aymeric, Jean Luc; Cami, Brigitte Gene, Vol. 106, Issue 1 https://doi.org/10.1016/0378-1119(91)90573-T	journal	September 1991
Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains. Kuhnert, P.; Hacker, J.; Mühldorfer, I. Applied and environmental microbiology, Vol. 63, Issue 2 https://doi.org/10.1128/AEM.63.2.703-709.1997	journal	January 1997
Characterization of a new endoglucanase from Erwinia chrysanthemi Boyer, Marie-Helene; Cami, Brigitte; Chambost, Jean-Pierre European Journal of Biochemistry, Vol. 162, Issue 2 https://doi.org/10.1111/j.1432-1033.1987.tb10602.x	journal	January 1987
The complete general secretory pathway in gram-negative bacteria. Pugsley, A. P. Microbiological Reviews, Vol. 57, Issue 1 https://doi.org/10.1128/MMBR.57.1.50-108.1993	journal	January 1993
Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose Beall, D. S.; Ingram, L. O. Journal of Industrial Microbiology, Vol. 11, Issue 3 https://doi.org/10.1007/BF01583716	journal	May 1993
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants Hueck, Christoph J. Microbiology and Molecular Biology Reviews, Vol. 62, Issue 2 https://doi.org/10.1128/MMBR.62.2.379-433.1998	journal	June 1998
Synergistic Interaction of Cellulases from Trichoderma reesei During Cellulose Degradation Nidetzky, Bernd; Steiner, Walter; Claeyssens, Marc ACS Symposium Series https://doi.org/10.1021/bk-1995-0618.ch008	book	May 1996
Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome. Pósfai, G.; Koob, M. D.; Kirkpatrick, H. A. Journal of Bacteriology, Vol. 179, Issue 13 https://doi.org/10.1128/JB.179.13.4426-4428.1997	journal	July 1997
Simultaneous expression of genes encoding endoglucanase and ?-glucosidase in Zymomonas mobilis Su, P.; Liu, C. -Q; Lucas, R. J. Biotechnology Letters, Vol. 15, Issue 9 https://doi.org/10.1007/BF00131768	journal	September 1993
Conversion of hydrolysates of corn cobs and hulls into ethanol by recombinantEscherichia coli B containing integrated genes for ethanol production Belll, D. S.; Ingram, L. O.; Ben-Bassat, A. Biotechnology Letters, Vol. 14, Issue 9 https://doi.org/10.1007/BF01029153	journal	September 1992
Analysis of eight out genes in a cluster required for pectic enzyme secretion by Erwinia chrysanthemi: sequence comparison with secretion genes from other gram-negative bacteria. Lindeberg, M.; Collmer, A. Journal of Bacteriology, Vol. 174, Issue 22 https://doi.org/10.1128/JB.174.22.7385-7397.1992	journal	January 1992
Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis Okamoto, T.; Yamano, S.; Ikeaga, H. Applied Microbiology and Biotechnology, Vol. 42, Issue 4 https://doi.org/10.1007/BF00173921	journal	December 1994
Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae Saito, Takao; Suzuki, Tohru; Hayashi, Akihiro Journal of Fermentation and Bioengineering, Vol. 69, Issue 5 https://doi.org/10.1016/0922-338X(90)90106-7	journal	January 1990
Conversion of xylan to ethanol by ethanologenic strains of Escherichia coli and Klebsiella oxytoca. Burchhardt, G.; Ingram, L. O. Applied and Environmental Microbiology, Vol. 58, Issue 4 https://doi.org/10.1128/AEM.58.4.1128-1133.1992	journal	January 1992
Economic Fundamentals of Ethanol Production from Lignocellulosic Biomass Wyman, Charles E. ACS Symposium Series https://doi.org/10.1021/bk-1995-0618.ch017	book	May 1996
Bioconversion for Production of Renewable Transportation Fuels in the United States Sheehan, John J. ACS Symposium Series https://doi.org/10.1021/bk-1994-0566.ch001	book	October 1994
Synergistic interaction of the Clostridium stercorarium cellulases Avicelase I (CelZ) and Avicelase II (CelY) in the degradation of microcrystalline cellulose Riedel, Kathrin; Ritter, Johannes; Bronnenmeier, Karin FEMS Microbiology Letters, Vol. 147, Issue 2 https://doi.org/10.1111/j.1574-6968.1997.tb10248.x	journal	February 1997

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