Methods for identifying an essential gene in a prokaryotic microorganism
Patent
·
OSTI ID:1175636
Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being transformed by artificial means and is capable of undergoing DNA recombination. This system offers an enhanced means of identifying essential function genes in diploid pathogens, such as gram-negative and gram-positive bacteria.
- Research Organization:
- California Institute of Technology, Pasadena, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG03-99ER62855
- Assignee:
- California Institute of Technology (Pasadena, CA)
- Patent Number(s):
- 6,991,900
- Application Number:
- 09/896,509
- OSTI ID:
- 1175636
- Country of Publication:
- United States
- Language:
- English
Construction and Characterization of a Human Bacterial Artificial Chromosome Library
|
journal | June 1996 |
The development and applications of the bacterial artificial chromosome cloning system.
|
journal | January 2001 |
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