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Advanced slow-magic angle spinning probe for magnetic resonance imaging and spectroscopy

Patent ·
OSTI ID:1175634
; ; ;
The present invention relates to a probe and processes useful for magnetic resonance imaging and spectroscopy instruments. More particularly, the invention relates to a MR probe and processes for obtaining resolution enhancements of fluid objects, including live specimens, using an ultra-slow (magic angle) spinning (MAS) of the specimen combined with a modified phase-corrected magic angle turning (PHORMAT) pulse sequence. Proton NMR spectra were measured of the torso and the top part of the belly of a female BALBc mouse in a 2T field, while spinning the animal at a speed of 1.5 Hz. Results show that even in this relatively low field with PHORMAT, an isotropic spectrum is obtained with line widths that are a factor 4.6 smaller than those obtained in a stationary mouse. Resolution of 1H NMR metabolite spectra are thus significantly enhanced. Results indicate that PHORMAT has the potential to significantly increase the utility of 1H NMR spectroscopy for in vivo biochemical, biomedical and/or medical applications involving large-sized biological objects such as mice, rats and even humans within a hospital setting. For small-sized objects, including biological objects, such as excised tissues, organs, live bacterial cells, and biofilms, use of PASS at a spinning rate of 30 Hz and above is preferred.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
6,989,674
Application Number:
10/639,828
OSTI ID:
1175634
Country of Publication:
United States
Language:
English

References (11)

Quantitation of intrinsic magnetic susceptibility-related effects in a tissue matrix. Phantom study journal March 1998
Removal of Dipolar Broadening of Nuclear Magnetic Resonance Spectra of Solids by Specimen Rotation journal June 1959
Quantitative localized 1H MR spectroscopy for clinical use journal September 1997
Gradient, high-resolution, magic angle spinning1H nuclear magnetic resonance spectroscopy of intact cells journal March 1998
Biochemical Analysis Using High-Resolution Magic Angle Spinning NMR Spectroscopy Distinguishes Lipoma-Like Well-Differentiated Liposarcoma from Normal Fat journal September 2001
High-resolution1H NMR and magic angle spinning NMR spectroscopic investigation of the biochemical effects of 2-bromoethanamine in intact renal and hepatic tissue journal January 2001
High-resolution1H NMR spectroscopy in rat liver using magic angle turning at a 1 Hz spinning rate journal April 2002
Measurements of Magnetic Field Variations in the Human Brain Using a 3D-FT Multiple Gradient Echo Technique journal February 1995
High-resolution1H NMR spectroscopy in organs and tissues using slow magic angle spinning journal January 2001
The evaluation of different MAS techniques at low spinning rates in aqueous samples and in the presence of magnetic susceptibility gradients journal November 2002
Magic-angle sample spinning of liquids journal August 1982

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