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Title: Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms

Abstract

A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175631
Patent Number(s):
6,989,252
Application Number:
09/748,690
Assignee:
Midwest Research Institute (Kansas City, MO) OSTI
DOE Contract Number:
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, and Seibert, Michael. Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms. United States: N. p., 2006. Web.
Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, & Seibert, Michael. Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms. United States.
Melis, Anastasios, Zhang, Liping, Benemann, John R., Forestier, Marc, Ghirardi, Maria, and Seibert, Michael. Tue . "Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms". United States. doi:. https://www.osti.gov/servlets/purl/1175631.
@article{osti_1175631,
title = {Hydrogen production using hydrogenase-containing oxygenic photosynthetic organisms},
author = {Melis, Anastasios and Zhang, Liping and Benemann, John R. and Forestier, Marc and Ghirardi, Maria and Seibert, Michael},
abstractNote = {A reversible physiological process provides for the temporal separation of oxygen evolution and hydrogen production in a microorganism, which includes the steps of growing a culture of the microorganism in medium under illuminated conditions to accumulate an endogenous substrate, depleting from the medium a nutrient selected from the group consisting of sulfur, iron, and/or manganese, sealing the culture from atmospheric oxygen, incubating the culture in light whereby a rate of light-induced oxygen production is equal to or less than a rate of respiration, and collecting an evolved gas. The process is particularly useful to accomplish a sustained photobiological hydrogen gas production in cultures of microorganisms, such as Chlamydomonas reinhardtii.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 24 00:00:00 EST 2006},
month = {Tue Jan 24 00:00:00 EST 2006}
}

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