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Title: Materials and methods for the alteration of enzyme and acetyl CoA levels in plants

Abstract

The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1.alpha. subunit of pPDH, the E1.beta. subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1.alpha. pPDH, E1.beta. pPDH, E2 pPDH,more » mtPDH or ALDH.« less

Inventors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Iowa State University Research Foundation, Inc., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175493
Patent Number(s):
6,942,994
Application Number:
10/293,865
Assignee:
Iowa State University Research Foundation, Inc. (Ames, IA) OSTI
DOE Contract Number:  
FC05-92OR22072
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Nikolau, Basil J., Wurtele, Eve S., Oliver, David J., Behal, Robert, Schnable, Patrick S., Ke, Jinshan, Johnson, Jerry L., Allred, Carolyn C., Fatland, Beth, Lutziger, Isabelle, and Wen, Tsui-Jung. Materials and methods for the alteration of enzyme and acetyl CoA levels in plants. United States: N. p., 2005. Web.
Nikolau, Basil J., Wurtele, Eve S., Oliver, David J., Behal, Robert, Schnable, Patrick S., Ke, Jinshan, Johnson, Jerry L., Allred, Carolyn C., Fatland, Beth, Lutziger, Isabelle, & Wen, Tsui-Jung. Materials and methods for the alteration of enzyme and acetyl CoA levels in plants. United States.
Nikolau, Basil J., Wurtele, Eve S., Oliver, David J., Behal, Robert, Schnable, Patrick S., Ke, Jinshan, Johnson, Jerry L., Allred, Carolyn C., Fatland, Beth, Lutziger, Isabelle, and Wen, Tsui-Jung. Tue . "Materials and methods for the alteration of enzyme and acetyl CoA levels in plants". United States. https://www.osti.gov/servlets/purl/1175493.
@article{osti_1175493,
title = {Materials and methods for the alteration of enzyme and acetyl CoA levels in plants},
author = {Nikolau, Basil J. and Wurtele, Eve S. and Oliver, David J. and Behal, Robert and Schnable, Patrick S. and Ke, Jinshan and Johnson, Jerry L. and Allred, Carolyn C. and Fatland, Beth and Lutziger, Isabelle and Wen, Tsui-Jung},
abstractNote = {The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1.alpha. subunit of pPDH, the E1.beta. subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1.alpha. pPDH, E1.beta. pPDH, E2 pPDH, mtPDH or ALDH.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 13 00:00:00 EDT 2005},
month = {Tue Sep 13 00:00:00 EDT 2005}
}

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