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Title: Apparatus and methods for high resolution separation of sample components on microfabricated channel devices

Abstract

Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through themore » turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.« less

Inventors:
; ; ;
Publication Date:
Research Org.:
The Regents of the University of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175416
Patent Number(s):
6,913,679
Application Number:
09/249,274
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
FG0391ER61125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Mathies, Richard A., Paegel, Brian, Simpson, Peter C., and Hutt, Lester. Apparatus and methods for high resolution separation of sample components on microfabricated channel devices. United States: N. p., 2005. Web.
Mathies, Richard A., Paegel, Brian, Simpson, Peter C., & Hutt, Lester. Apparatus and methods for high resolution separation of sample components on microfabricated channel devices. United States.
Mathies, Richard A., Paegel, Brian, Simpson, Peter C., and Hutt, Lester. Tue . "Apparatus and methods for high resolution separation of sample components on microfabricated channel devices". United States. https://www.osti.gov/servlets/purl/1175416.
@article{osti_1175416,
title = {Apparatus and methods for high resolution separation of sample components on microfabricated channel devices},
author = {Mathies, Richard A. and Paegel, Brian and Simpson, Peter C. and Hutt, Lester},
abstractNote = {Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through the turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2005},
month = {7}
}

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