skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods for simultaneous control of lignin content and composition, and cellulose content in plants

Patent ·
OSTI ID:1175241

The present invention relates to a method of concurrently introducing multiple genes into plants and trees is provided. The method includes simultaneous transformation of plants with multiple genes from the phenylpropanoid pathways including 4CL, CAld5H, AldOMT, SAD and CAD genes and combinations thereof to produce various lines of transgenic plants displaying altered agronomic traits. The agronomic traits of the plants are regulated by the orientation of the specific genes and the selected gene combinations, which are incorporated into the plant genome.

Research Organization:
Board of Control of Michigan Technological University, Houghton, MI (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-01ER15179
Assignee:
Board of Control of Michigan Technological University (Houghton, MI)
Patent Number(s):
6,855,864
Application Number:
09/947,027
OSTI ID:
1175241
Country of Publication:
United States
Language:
English

References (68)

Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees journal August 1999
Down-regulation of Cinnamoyl-CoA Reductase induces significant changes of lignin profiles in transgenic tobacco plants journal January 1998
Immunological characterization of transgenic tobacco plants with a chimeric gene for 4-coumarate:CoA ligase that have altered lignin in their xylem tissue journal September 1997
Expression of a soybean ?-conclycinin gene under the control of the Cauliflower Mosaic Virus 35S and 19S promoters in transformed petunia tissues journal January 1987
Assaying chimeric genes in plants: The GUS gene fusion system journal December 1987
DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene journal October 1987
Characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene. journal August 1992
Expression of Foreign Genes in Transgenic Yellow-Poplar Plants journal January 1992
Lignin Constituents and Cell‐Wall Digestibility of Grass and Legume Stems journal May 1988
The Difference between Guaiacyl and Guaiacyl-Syringyl Lignins in their Responses to Kraft Delignification journal January 1990
Insertion of the Maize Transposable Element AC Into Poplar book January 1991
Structure and expression of the maize gene encoding the phosphoenolpyruvate carboxylase isozyme involved in C4 photosynthesis journal May 1989
Characterization of bispecific caffeic acid/ 5-hydroxyferulic acid O-methyltransferase from aspen journal May 1992
Analyses of Genotypic Diversity among North, South, and Central American Isolates of Sugarcane Yellow Leaf Virus: Evidence for Colombian Origins and for Intraspecific Spatial Phylogenetic Variation journal February 2002
Pilus Assembly by Agrobacterium T-DNA Transfer Genes journal August 1996
Stable genetic transformation of white pine (Pinus strobus L.) after cocultivation of embryogenic tissues with Agrobacterium tumefaciens journal October 1999
Maize sucrose synthase-1 promoter directs phloem cell-specific expression of Gus gene in transgenic tobacco plants journal June 1990
Identification of an essential upstream element in the nopaline synthase promoter by stable and transient assays journal August 1987
Trends in lignin modification: a comprehensive analysis of the effects of genetic manipulations/mutations on lignification and vascular integrity journal October 2002
Plant O-methyl-transferase signatures journal July 1997
Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus journal June 1992
Secondary xylem-specific expression of caffeoyl-coenzyme A 3-O-methyltransferase plays an important role in the methylation pathway associated with lignin biosynthesis in loblolly pine journal July 1999
Conserved sequence motifs in plant S-adenosyl-L-methionine-dependent methyltransferases journal January 1998
An alternative approach for gene transfer in trees using wild-typeAgrobacterium strains journal September 1991
Evidence for a novel biosynthetic pathway that regulates the ratio of syringyl to guaiacyl residues in lignin in the differentiating xylem of Magnolia kobus DC journal February 1999
Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases journal September 1987
A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation journal July 1983
Expression of the mutantArabidopsis thaliana acetolactate synthase gene confers chlorsulfuron resistance to transgenic poplar plants journal May 1992
Two Regulatory Genes of the Maize Anthocyanin Pathway Are Homologous: Isolation of B Utilizing R Genomic Sequences journal December 1989
Factors Influencing the Tissue Culture and the Agrobacterium tumefaciens -Mediated Transformation of Hybrid Aspen and Poplar Clones journal July 1990
Transformation of Liquidambar styraciflua using Agrobacterium tumefaciens journal April 1993
Substrate preferences of O-methyltransferases in alfalfa suggest new pathways for 3-O-methylation of monolignols journal January 2001
Agrobacterium-Mediated Transformation of Walnut Somatic Embryos and Regeneration of Transgenic Plants journal July 1988
Suppression of O -Methyltransferase Gene by Homologous Sense Transgene in Quaking Aspen Causes Red-Brown Wood Phenotypes journal May 1998
Alterations in the Biosynthesis of Lignin in Transgenic Plants with Chimeric Genes for 4-Coumarate: Coenzyme A Ligase journal October 1996
cDNA cloning, sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen journal December 1991
Inheritance, gene expression, and lignin characterization in a mutant pine deficient in cinnamyl alcohol dehydrogenase journal July 1997
A general method applicable to the search for similarities in the amino acid sequence of two proteins journal March 1970
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter journal February 1985
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. journal April 1990
Molecular Cloning of 4-Coumarate:Coenzyme A Ligase in Loblolly Pine and the Roles of This Enzyme in the Biosynthesis of Lignin in Compression Wood journal January 1997
The Last Step of Syringyl Monolignol Biosynthesis in Angiosperms Is Regulated by a Novel Gene Encoding Sinapyl Alcohol Dehydrogenase journal July 2001
Importance of the Proline-Rich Region Following Signal-Anchor Sequence in the Formation of Correct Conformation of Microsomal Cytochrome P-450s1 journal November 1993
Improved efficiency of the walnut somatic embryo gene transfer system journal January 1990
P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature journal January 1996
Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer:Larix decidua journal October 1991
Transgenic poplars: expression of chimeric genes using four different constructs journal April 1992
A xylem-specific cellulose synthase gene from aspen (Populus tremuloides) is responsive to mechanical stress journal June 2000
A Simple and General Method for Transferring Genes into Plants journal March 1985
5-Hydroxyconiferyl Aldehyde Modulates Enzymatic Methylation for Syringyl Monolignol Formation, a New View of Monolignol Biosynthesis in Angiosperms journal February 2000
Lignins: Occurrence, formation, structure and reactions, K. V. Sarkanen and C. H. Ludwig, Eds., John Wiley & Sons, Inc., New York, 1971. 916 pp. $35.00 journal March 1972
Biochemistry and molecular biology of lignification journal February 1995
Differences in Induction of Ti Plasmid Virulence Genes virG and virD , and Continued Control of virD Expression by Four External Factors journal January 1989
Segregation of transgenes in maize journal January 1992
Transformation of Poplar by Agrobacterium tumefaciens journal June 1986
Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms journal August 1999
An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation journal June 1988
Influence of Lignin on Digestibility of Forage Cell Wall Material journal June 1986
Production of fertile transgenic maize by electroporation of suspension culture cells journal January 1994
Selection of marker-free transgenic plants using the isopentenyl transferase gene journal March 1997
Increased Virulence of Agrobacterium Rhizogenes Conferred by the vir Region of pTiBo542: Application to Genetic Engineering of Poplar journal December 1987
Lignin: Its Constitution and Formation from p-Hydroxycinnamyl Alcohols: Lignin is duplicated by dehydrogenation of these alcohols; intermediates explain formation and structure journal April 1965
Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides) journal April 1998
Agrobacterium mediated transformation and regeneration of Populus journal February 1987
Fas ligand—a double-edged sword journal November 1998
Spatial pattern of cauliflower mosaic virus 35S promoter-luciferase expression in transgenic hybrid aspen trees monitored by enzymatic assay and non-destructive imaging journal September 1992
Transgenic Populus hybrid expresses a wound-inducible potato proteinase inhibitor II - CAT gene fusion journal September 1991
Genetic analysis of cinnamyl alcohol dehydrogenase in loblolly pine: single gene inheritance, molecular characterization and evolution journal September 1995

Similar Records

Final Report on Regulation of Guaiacyl and Syringyl Monolignol Biosynthesis
Technical Report · Thu Mar 09 00:00:00 EST 2006 · OSTI ID:1175241

Genetic engineering of syringyl-enriched lignin in plants
Patent · Tue Nov 02 00:00:00 EST 2004 · OSTI ID:1175241

Genetic dissection of bioenerrgy traits in sorghum
Technical Report · Fri Jun 15 00:00:00 EDT 2012 · OSTI ID:1175241

Related Subjects