Plant nitrogen regulatory P-PII polypeptides
The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the invention may be used to engineer organisms to overexpress wild-type or mutant P-PII regulatory protein. Engineered plants that overexpress or underexpress P-PII regulatory protein may have increased nitrogen assimilation capacity. Engineered organisms may be used to produce P-PII proteins which, in turn, can be used for a variety of purposes including in vitro screening of herbicides. P-PII nucleotide sequences have additional uses as probes for isolating additional genomic clones having the promoters of P-PII gene. P-PII promoters are light- and/or sucrose-inducible and may be advantageously used in genetic engineering of plants.
- Research Organization:
- New York University, New York, NY (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-94ER20133
- Assignee:
- New York University (New York, NY)
- Patent Number(s):
- 6,822,079
- Application Number:
- 09/756,541
- OSTI ID:
- 1175148
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expansin polynucleotides, related polypeptides and methods of use
Gene encoding plant asparagine synthetase