Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

Title: Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

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Abstract

Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

Inventors:: Glazer, Alexander N.; Tooley, Aaron J.; Cai, Yuping

Publication Date:: 2004-05-25

Research Org.:: Univ. of California, Oakland, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1174863

Patent Number(s):: 6,740,507

Application Number:: 09/919,486

Assignee:: University Of California, The Regents Of

DOE Contract Number:: FG-9161125

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs.  United States: N. p., 2004. 
        Web.

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                    Glazer, Alexander N., Tooley, Aaron J., & Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs.  United States.

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                    Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Tue .  
        "Engineering of living cells for the expression of holo-phycobiliprotein-based constructs".  United States.  https://www.osti.gov/servlets/purl/1174863.

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@article{osti_1174863,

  title        = {Engineering of living cells for the expression of holo-phycobiliprotein-based constructs},

  author       = {Glazer, Alexander N. and Tooley, Aaron J. and Cai, Yuping},

  abstractNote = {Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/1174863},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2004},

  month        = {5}

}

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