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Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

Patent ·
OSTI ID:1174863
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Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
Research Organization:
The Regents of the University of California, Oakland, CA (United States)
Sponsoring Organization:
USDOE
Assignee:
University Of California, The Regents Of
Patent Number(s):
6,740,507
Application Number:
09/919,486
OSTI ID:
1174863
Country of Publication:
United States
Language:
English

References (6)

Genetic engineering of phytochrome biosynthesis in bacteria journal September 2001
Functional Genomic Analysis of the HY2 Family of Ferredoxin-Dependent Bilin Reductases from Oxygenic Photosynthetic Organisms journal April 2001
Recombinant holophytochrome in Escherichia coli journal November 2001
Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-  subunit in a heterologous host journal September 2001
The use of phycobiliproteins as fluorescent labels in immunoassay journal August 1986
Phycocyanin alpha-subunit phycocyanobilin lyase. journal August 1992

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