PCR amplification on microarrays of gel immobilized oligonucleotides

Strizhkov, Boris; Tillib, Sergei; Mikhailovich, Vladimir; Mirzabekov, Andrei

Title: PCR amplification on microarrays of gel immobilized oligonucleotides

Patent · Tue Nov 04 00:00:00 EST 2003

OSTI ID:1174559

Strizhkov, Boris; Tillib, Sergei; Mikhailovich, Vladimir; Mirzabekov, Andrei

The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.

View Patent

Cite

Export

Save

Research Organization:: Univ. of Chicago, IL (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-31-1-0-ENG-39

Assignee:: University Of Chicago

Patent Number(s):: 6,642,000

Application Number:: 09/559,306

OSTI ID:: 1174559

Country of Publication:: United States

Language:: English

References (7)

Immobilization of DNA in Polyacrylamide Gel for the Manufacture of DNA and DNA–Oligonucleotide Microchips Proudnikov, Dmitri; Timofeev, Edward; Mirzabekov, Andrei Analytical Biochemistry, Vol. 259, Issue 1 https://doi.org/10.1006/abio.1998.2620	journal	May 1998
Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization Dubiley, S.; Kirillov, E.; Lysov, Y. Nucleic Acids Research, Vol. 25, Issue 12 https://doi.org/10.1093/nar/25.12.2259	journal	June 1997
Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips Fotin, A. V.; Drobyshev, A. L.; Proudnikov, D. Y. Nucleic Acids Research, Vol. 26, Issue 6 https://doi.org/10.1093/nar/26.6.1515	journal	March 1998
Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip Waters, Larry C.; Jacobson, Stephen C.; Kroutchinina, Natalia Analytical Chemistry, Vol. 70, Issue 24 https://doi.org/10.1021/ac980447e	journal	December 1998
Protein Microchips: Use for Immunoassay and Enzymatic Reactions Arenkov, Pavel; Kukhtin, Alexander; Gemmell, Anne Analytical Biochemistry, Vol. 278, Issue 2 https://doi.org/10.1006/abio.1999.4363	journal	February 2000
DNA analysis and diagnostics on oligonucleotide microchips. Yershov, G.; Barsky, V.; Belgovskiy, A. Proceedings of the National Academy of Sciences, Vol. 93, Issue 10 https://doi.org/10.1073/pnas.93.10.4913	journal	May 1996
Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers Dubiley, S. Nucleic Acids Research, Vol. 27, Issue 18 https://doi.org/10.1093/nar/27.18.e19	journal	September 1999

Similar Records

PCR amplfication on a microarray of gel-immobilized oligonucleotides : detection of bacterial toxin- and drug-resistent genes and their mutations.

Journal Article · Sun Oct 01 00:00:00 EDT 2000 · Biotech. · OSTI ID:1174559

Strizhkov, B N; Drobyshev, A L; Mikhailovich, V M; +1 more

Integration of multiple PCR amplification and DNA mutation analyses by using oligonucleotide microchip.

Journal Article · Tue May 01 00:00:00 EDT 2001 · Anal. Biochem. · OSTI ID:1174559

Tillib, S V; Strizhkov, B N; Mirzabekov, A D

Fabrication of microarray of gel-immobilized compounds on a chip by copolymerization.

Journal Article · Wed Sep 01 00:00:00 EDT 1999 · Biotech. · OSTI ID:1174559

Vasiliskov, A V; Timofeev, E N; Surzhikov, S A; +3 more

Related Subjects

47 OTHER INSTRUMENTATION
42 ENGINEERING

Title: PCR amplification on microarrays of gel immobilized oligonucleotides

Citation Formats

References (7)

Similar Records

Related Subjects