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Title: Methods for determining the physiological state of a plant

Patent ·
OSTI ID:1174506

The present invention provides methods for measuring a photosynthetic parameter. The methods of the invention include the steps of: (a) illuminating a plant leaf until steady-state photosynthesis is achieved; (b) subjecting the illuminated plant leaf to a period of darkness; (c) using a kinetic spectrophotometer or kinetic spectrophotometer/fluorimeter to collect spectral data from the plant leaf treated in accordance with steps (a) and (b); and (d) determining a photosynthetic parameter from the spectral data. In another aspect, the invention provides methods for determining the physiological state of a plant.

Research Organization:
Washington State University Research Foundation, Pullman, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FGO3-98ER20299
Assignee:
Washington State University Research Foundation
Patent Number(s):
6,624,887
Application Number:
09/642,385
OSTI ID:
1174506
Country of Publication:
United States
Language:
English

References (7)

Compound parabolic concentrator probe for efficient light collection in spectroscopy of biological tissue journal January 1996
Control and Measurement of Photosynthetic Electron Transport in Vivo book
The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves journal September 1990
Activation of the chloroplast ATPase measured by the electrochromic change in leaves of intact plants journal August 1989
A portable multi-flash kinetic fluorimeter for measurement of donor and acceptor reactions of Photosystem 2 in leaves of intact plants under field conditions journal December 1990
H+/E- Ratios for Photosynthetic Electron Transfer in Intact Leaves in the Steady State. book January 1998
Dissipation of the Proton Electrochemical Potential in Intact and Lysed Chloroplasts: I. The Electrical Potential journal February 1991

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