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Title: Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function

Abstract

Method and apparatus for examining biological materials using diffuse reflectance spectroscopy and the Kubelka-Munk function. In one aspect, the method is used to determine whether a tissue sample is cancerous or not and comprises the steps of (a) measuring the diffuse reflectance from the tissue sample at a first wavelength and at a second wavelength, wherein the first wavelength is a wavelength selected from the group consisting of 255-265 nm and wherein the second wavelength is a wavelength selected from the group consisting of 275-285 nm; (b) using the Kubelka-Munk function to transform the diffuse reflectance measurement obtained at the first and second wavelengths; and (c) comparing a ratio or a difference of the transformed Kubelka-Munk measurements at the first and second wavelengths to appropriate standards determine whether or not the tissue sample is cancerous. One can use the spectral profile of KMF between 250 nm to 300 nm to determine whether or not the tissue sample is cancerous or precancerous. According to the value at the first and second wavelengths determine whether or not the malignant tissue is invasive or mixed invasive and in situ or carcinoma in situ.

Inventors:
;
Publication Date:
Research Org.:
The Research Foundation of CUNY, New York, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174478
Patent Number(s):
6,615,068
Application Number:
09/598,007
Assignee:
The Research Foundation of CUNY (New York, NY) OSTI
DOE Contract Number:  
FG02-98ER62560
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE

Citation Formats

Alfano, Robert R., and Yang, Yuanlong. Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function. United States: N. p., 2003. Web.
Alfano, Robert R., & Yang, Yuanlong. Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function. United States.
Alfano, Robert R., and Yang, Yuanlong. Tue . "Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function". United States. https://www.osti.gov/servlets/purl/1174478.
@article{osti_1174478,
title = {Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function},
author = {Alfano, Robert R. and Yang, Yuanlong},
abstractNote = {Method and apparatus for examining biological materials using diffuse reflectance spectroscopy and the Kubelka-Munk function. In one aspect, the method is used to determine whether a tissue sample is cancerous or not and comprises the steps of (a) measuring the diffuse reflectance from the tissue sample at a first wavelength and at a second wavelength, wherein the first wavelength is a wavelength selected from the group consisting of 255-265 nm and wherein the second wavelength is a wavelength selected from the group consisting of 275-285 nm; (b) using the Kubelka-Munk function to transform the diffuse reflectance measurement obtained at the first and second wavelengths; and (c) comparing a ratio or a difference of the transformed Kubelka-Munk measurements at the first and second wavelengths to appropriate standards determine whether or not the tissue sample is cancerous. One can use the spectral profile of KMF between 250 nm to 300 nm to determine whether or not the tissue sample is cancerous or precancerous. According to the value at the first and second wavelengths determine whether or not the malignant tissue is invasive or mixed invasive and in situ or carcinoma in situ.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {9}
}

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