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Title: Study of polymer molecules and conformations with a nanopore

Abstract

The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.

Inventors:
; ; ;
Publication Date:
Research Org.:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171692
Patent Number(s):
8,969,091
Application Number:
13/766,231
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
DOE Contract Number:  
FG02-01ER45922
Resource Type:
Patent
Resource Relation:
Patent File Date: 2013 Feb 13
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Golovchenko, Jene A, Li, Jiali, Stein, Derek, and Gershow, Marc H. Study of polymer molecules and conformations with a nanopore. United States: N. p., 2015. Web.
Golovchenko, Jene A, Li, Jiali, Stein, Derek, & Gershow, Marc H. Study of polymer molecules and conformations with a nanopore. United States.
Golovchenko, Jene A, Li, Jiali, Stein, Derek, and Gershow, Marc H. Tue . "Study of polymer molecules and conformations with a nanopore". United States. doi:. https://www.osti.gov/servlets/purl/1171692.
@article{osti_1171692,
title = {Study of polymer molecules and conformations with a nanopore},
author = {Golovchenko, Jene A and Li, Jiali and Stein, Derek and Gershow, Marc H},
abstractNote = {The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 03 00:00:00 EST 2015},
month = {Tue Mar 03 00:00:00 EST 2015}
}

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Works referenced in this record:

Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA Molecules
journal, December 1999


Sequencing and the Single Channel
journal, December 1999


Stochastic sensors inspired by biology
journal, September 2001

  • Bayley, Hagan; Cremer, Paul S.
  • Nature, Vol. 413, Issue 6852, p. 226-230
  • DOI: 10.1038/35093038

A common receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B
journal, September 1973

  • Braun, Volkmar; Schaller, Klaus; Wolff, Helga
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 323, Issue 1, p. 87-97
  • DOI: 10.1016/0005-2736(73)90433-1

Effect of point mutations on the in-vitro pore properties of maltoporin, a protein of Escherichia coli outer membrane
journal, June 1988

  • Dargent, Bénédicte; Charbit, Alain; Hofnung, Maurice
  • Journal of Molecular Biology, Vol. 201, Issue 3, p. 497-506
  • DOI: 10.1016/0022-2836(88)90632-8

Selectivity for maltose and maltodextrins of maltoporin, a pore-forming protein of E. coli outer membrane
journal, August 1987


Nanopores and nucleic acids: prospects for ultrarapid sequencing
journal, April 2000


Electrokinetic measurements with submicron particles and pores by the resistive pulse technique
journal, September 1977

  • DeBlois, Ralph W.; Bean, Charles P.; Wesley, Roy K. A.
  • Journal of Colloid and Interface Science, Vol. 61, Issue 2, p. 323-335
  • DOI: 10.1016/0021-9797(77)90395-2

Alamethicin. A rich model for channel behavior
journal, January 1984


Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels
journal, October 1988


Open channel noise. V. Fluctuating barriers to ion entry in gramicidin A channels
journal, March 1990


Two types of inactivation in Shaker K+ channels: Effects of alterations in the carboxy-terminal region
journal, October 1991


Channeling phage DNA through membranes: from in vivo to in vitro
journal, May 2003

  • Letellier, Lucienne; Boulanger, Pascale; de Frutos, Marta
  • Research in Microbiology, Vol. 154, Issue 4, p. 283-287
  • DOI: 10.1016/S0923-2508(03)00072-X

Ion-beam sculpting at nanometre length scales
journal, July 2001

  • Li, Jiali; Stein, Derek; McMullan, Ciaran
  • Nature, Vol. 412, Issue 6843, p. 166-169
  • DOI: 10.1038/35084037

Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in shaker K+ channels
journal, August 1991


Improved stability of black lipid membranes by coating with polysaccharide derivatives bearing hydrophobic anchor groups
journal, May 1986

  • Moellerfeld, J.; Prass, W.; Ringsdorf, H.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 857, Issue 2, p. 265-270
  • DOI: 10.1016/0005-2736(86)90355-X

Open channel noise. III. High-resolution recordings show rapid current fluctuations in gramicidin A and four chemical analogues
journal, December 1987


A protein-conducting channel in the endoplasmic reticulum
journal, May 1991


Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores
journal, November 1992


"Reversed" alamethicin conductance in lipid bilayers
journal, April 1991


Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps
journal, August 1990


Aqueous Stabilization and Self-Assembly of Graphene Sheets into Layered Bio-Nanocomposites using DNA
journal, August 2009

  • Patil, Avinash J.; Vickery, Jemma L.; Scott, Thomas B.
  • Advanced Materials, Vol. 21, Issue 31, p. 3159-3164
  • DOI: 10.1002/adma.200803633