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Title: Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation

Abstract

In this project, we combined molecular genetic, spectroscopic, and microscopic techniques with kinetic and reactive transport studies to describe and quantify biotic and abiotic mechanisms underlying anaerobic, nitrate-dependent U(IV) and Fe(II) oxidation, which influences the long-term efficacy of in situ reductive immobilization of uranium at DOE sites. In these studies, Thiobacillus denitrificans, an autotrophic bacterium that catalyzes anaerobic U(IV) and Fe(II) oxidation, was used to examine coupled oxidation-reduction processes under either biotic (enzymatic) or abiotic conditions in batch and column experiments with biogenically produced UIVO2(s). Synthesis and quantitative analysis of coupled chemical and transport processes were done with the reactive transport modeling code Crunchflow. Research focused on identifying the primary redox proteins that catalyze metal oxidation, environmental factors that influence protein expression, and molecular-scale geochemical factors that control the rates of biotic and abiotic oxidation.

Authors:
 [1];  [1];  [1];  [2];  [2];  [2]
  1. Univ. of California, Merced, CA (United States)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Univ. of California, Merced, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1171384
Report Number(s):
DOE-UCM-BioU
DOE Contract Number:
SC0005479
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; Uranium; anaerobic oxidation; kinetics; reactive transport modeling; X-ray absorption spectroscopy; Thiobacillus denitrificans

Citation Formats

O'Day, Peggy A., Asta, Maria P., Kanematsu, Masakazu, Beller, Harry, Zhou, Peng, and Steefel, Carl. Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation. United States: N. p., 2015. Web. doi:10.2172/1171384.
O'Day, Peggy A., Asta, Maria P., Kanematsu, Masakazu, Beller, Harry, Zhou, Peng, & Steefel, Carl. Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation. United States. doi:10.2172/1171384.
O'Day, Peggy A., Asta, Maria P., Kanematsu, Masakazu, Beller, Harry, Zhou, Peng, and Steefel, Carl. Fri . "Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation". United States. doi:10.2172/1171384. https://www.osti.gov/servlets/purl/1171384.
@article{osti_1171384,
title = {Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation},
author = {O'Day, Peggy A. and Asta, Maria P. and Kanematsu, Masakazu and Beller, Harry and Zhou, Peng and Steefel, Carl},
abstractNote = {In this project, we combined molecular genetic, spectroscopic, and microscopic techniques with kinetic and reactive transport studies to describe and quantify biotic and abiotic mechanisms underlying anaerobic, nitrate-dependent U(IV) and Fe(II) oxidation, which influences the long-term efficacy of in situ reductive immobilization of uranium at DOE sites. In these studies, Thiobacillus denitrificans, an autotrophic bacterium that catalyzes anaerobic U(IV) and Fe(II) oxidation, was used to examine coupled oxidation-reduction processes under either biotic (enzymatic) or abiotic conditions in batch and column experiments with biogenically produced UIVO2(s). Synthesis and quantitative analysis of coupled chemical and transport processes were done with the reactive transport modeling code Crunchflow. Research focused on identifying the primary redox proteins that catalyze metal oxidation, environmental factors that influence protein expression, and molecular-scale geochemical factors that control the rates of biotic and abiotic oxidation.},
doi = {10.2172/1171384},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Feb 27 00:00:00 EST 2015},
month = {Fri Feb 27 00:00:00 EST 2015}
}

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