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Title: A Method to determine lysine acetylation stoichiometries

Abstract

A major bottleneck to fully understanding the functional aspects of lysine acetylation is the lack of stoichiometry information. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of lysine acetylation on proteins globally. Using this technique, we determined the modification occupancy on hundreds of acetylated peptides from cell lysates and cross-validated the measurements via immunoblotting.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171295
Report Number(s):
PNNL-SA-96571
42294; 400412000
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Proteomics, 2014:Article No. 730725
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Nakayasu, Ernesto S., Wu, Si, Sydor, Michael A., Shukla, Anil K., Weitz, Karl K., Moore, Ronald J., Hixson, Kim K., Kim, Jong Seo, Petyuk, Vladislav A., Monroe, Matthew E., Pasa-Tolic, Ljiljana, Qian, Weijun, Smith, Richard D., Adkins, Joshua N., and Ansong, Charles. A Method to determine lysine acetylation stoichiometries. United States: N. p., 2014. Web. doi:10.1155/2014/730725.
Nakayasu, Ernesto S., Wu, Si, Sydor, Michael A., Shukla, Anil K., Weitz, Karl K., Moore, Ronald J., Hixson, Kim K., Kim, Jong Seo, Petyuk, Vladislav A., Monroe, Matthew E., Pasa-Tolic, Ljiljana, Qian, Weijun, Smith, Richard D., Adkins, Joshua N., & Ansong, Charles. A Method to determine lysine acetylation stoichiometries. United States. doi:10.1155/2014/730725.
Nakayasu, Ernesto S., Wu, Si, Sydor, Michael A., Shukla, Anil K., Weitz, Karl K., Moore, Ronald J., Hixson, Kim K., Kim, Jong Seo, Petyuk, Vladislav A., Monroe, Matthew E., Pasa-Tolic, Ljiljana, Qian, Weijun, Smith, Richard D., Adkins, Joshua N., and Ansong, Charles. Mon . "A Method to determine lysine acetylation stoichiometries". United States. doi:10.1155/2014/730725.
@article{osti_1171295,
title = {A Method to determine lysine acetylation stoichiometries},
author = {Nakayasu, Ernesto S. and Wu, Si and Sydor, Michael A. and Shukla, Anil K. and Weitz, Karl K. and Moore, Ronald J. and Hixson, Kim K. and Kim, Jong Seo and Petyuk, Vladislav A. and Monroe, Matthew E. and Pasa-Tolic, Ljiljana and Qian, Weijun and Smith, Richard D. and Adkins, Joshua N. and Ansong, Charles},
abstractNote = {A major bottleneck to fully understanding the functional aspects of lysine acetylation is the lack of stoichiometry information. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of lysine acetylation on proteins globally. Using this technique, we determined the modification occupancy on hundreds of acetylated peptides from cell lysates and cross-validated the measurements via immunoblotting.},
doi = {10.1155/2014/730725},
journal = {International Journal of Proteomics, 2014:Article No. 730725},
number = ,
volume = ,
place = {United States},
year = {Mon Jul 21 00:00:00 EDT 2014},
month = {Mon Jul 21 00:00:00 EDT 2014}
}