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Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Abstract

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.

Inventors:
;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171090
Patent Number(s):
8,962,325
Application Number:
12/242,474
Assignee:
The Regents of the University of California (Oakland, CA) CHO
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Sep 30
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Stampfer, Martha R, and Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States: N. p., 2015. Web.
Stampfer, Martha R, & Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States.
Stampfer, Martha R, and Garbe, James C. Tue . "Increasing cell culture population doublings for long-term growth of finite life span human cell cultures". United States. doi:. https://www.osti.gov/servlets/purl/1171090.
@article{osti_1171090,
title = {Increasing cell culture population doublings for long-term growth of finite life span human cell cultures},
author = {Stampfer, Martha R and Garbe, James C},
abstractNote = {Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 24 00:00:00 EST 2015},
month = {Tue Feb 24 00:00:00 EST 2015}
}

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Works referenced in this record:

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Telomerase protects developing neurons against DNA damage-induced cell death
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Long-term culture of adult murine epidermal keratinocytes
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