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Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Patent ·
OSTI ID:1171090
;
Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
8,962,325
Application Number:
12/242,474
OSTI ID:
1171090
Country of Publication:
United States
Language:
English

References (14)

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Increased epidermal tumors and increased skin wound healing in transgenic mice overexpressing the catalytic subunit of telomerase, mTERT, in basal keratinocytes journal June 2001
Oxytocin and Oxytocin Receptors in Cancer Cells and Proliferation journal April 2004
Long-term culture of adult murine epidermal keratinocytes journal December 2005
Absence of cancer–associated changes in human fibroblasts immortalized with telomerase journal January 1999
Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells journal April 2001
Telomerase protects developing neurons against DNA damage-induced cell death journal November 2001
Increased p16 expression with first senescence arrest in human mammary epithelial cells and extended growth capacity with p16 inactivation journal July 1998
Telomerase beyond telomeres journal August 2002
Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival journal August 2001
Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells journal November 2002
Oxytocin enhances myoepithelial cell differentiation and proliferation in the mouse mammary gland. journal August 1993
Telomerase Maintains Telomere Structure in Normal Human Cells journal July 2003

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