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Title: Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Abstract

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

Inventors:
; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171088
Patent Number(s):
8,962,346
Application Number:
13/423,050
Assignee:
Sandia Corporation (Albuquerque, NM) SSO
DOE Contract Number:
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Mar 16
Country of Publication:
United States
Language:
English
Subject:
42 ENGINEERING

Citation Formats

Schaff, Ulrich Y, Koh, Chung-Yan, and Sommer, Gregory J. Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation. United States: N. p., 2015. Web.
Schaff, Ulrich Y, Koh, Chung-Yan, & Sommer, Gregory J. Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation. United States.
Schaff, Ulrich Y, Koh, Chung-Yan, and Sommer, Gregory J. Tue . "Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation". United States. doi:. https://www.osti.gov/servlets/purl/1171088.
@article{osti_1171088,
title = {Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation},
author = {Schaff, Ulrich Y and Koh, Chung-Yan and Sommer, Gregory J},
abstractNote = {Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 24 00:00:00 EST 2015},
month = {Tue Feb 24 00:00:00 EST 2015}
}

Patent:

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