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Title: Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva

Abstract

Dermacentor andersoni, known as the Rocky Mountain wood tick, is found in the western United States and transmits pathogens that cause diseases of veterinary and public health importance including Rocky Mountain spotted fever, tularemia, Colorado tick fever and bovine anaplasmosis. Tick saliva is known to modulate both innate and acquired immune responses, enabling ticks to feed for several days without detection. During feeding ticks subvert host defences such as hemostasis and inflammation, which would otherwise result in coagulation, wound repair and rejection of the tick. Molecular characterization of the proteins and pharmacological molecules secreted in tick saliva offers an opportunity to develop tick vaccines as an alternative to the use of acaricides, as well as new anti-inflammatory drugs. We performed proteomics informed by transcriptomics to identify D. andersoni saliva proteins that are secreted during feeding. The transcript data generated a database of 21,797 consensus sequences, which we used to identify 677 proteins secreted in the saliva of D. andersoni ticks fed for 2 and 5 days, following proteomic investigations of whole saliva using mass spectrometry. Salivary gland transcript levels of unfed ticks were compared with 2 and 5 day fed ticks to identify genes upregulated early during tick feeding. Wemore » cross-referenced the proteomic data with the transcriptomic data to identify 157 proteins of interest for immunomodulation and blood feeding. Proteins of unknown function as well as known immunomodulators were identified.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1170475
Report Number(s):
PNNL-SA-105510
47421; KP1704020
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal for Parasitology, 44(13):1029-1037
Country of Publication:
United States
Language:
English
Subject:
Proteomics; transcriptomics; Dermacentor andersoni; saliva; Environmental Molecular Sciences Laboratory

Citation Formats

Mudenda, Lwiindi, Aguilar Pierle, Sebastian, Turse, Joshua E., Scoles, Glen A., Purvine, Samuel O., Nicora, Carrie D., Clauss, Therese RW, Ueti, Massaro W., Brown, Wendy C., and Brayton, Kelly A.. Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva. United States: N. p., 2014. Web. doi:10.1016/j.ijpara.2014.07.003.
Mudenda, Lwiindi, Aguilar Pierle, Sebastian, Turse, Joshua E., Scoles, Glen A., Purvine, Samuel O., Nicora, Carrie D., Clauss, Therese RW, Ueti, Massaro W., Brown, Wendy C., & Brayton, Kelly A.. Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva. United States. doi:10.1016/j.ijpara.2014.07.003.
Mudenda, Lwiindi, Aguilar Pierle, Sebastian, Turse, Joshua E., Scoles, Glen A., Purvine, Samuel O., Nicora, Carrie D., Clauss, Therese RW, Ueti, Massaro W., Brown, Wendy C., and Brayton, Kelly A.. Thu . "Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva". United States. doi:10.1016/j.ijpara.2014.07.003.
@article{osti_1170475,
title = {Proteomics informed by transcriptomics identifies novel secreted proteins in Dermacentor andersoni saliva},
author = {Mudenda, Lwiindi and Aguilar Pierle, Sebastian and Turse, Joshua E. and Scoles, Glen A. and Purvine, Samuel O. and Nicora, Carrie D. and Clauss, Therese RW and Ueti, Massaro W. and Brown, Wendy C. and Brayton, Kelly A.},
abstractNote = {Dermacentor andersoni, known as the Rocky Mountain wood tick, is found in the western United States and transmits pathogens that cause diseases of veterinary and public health importance including Rocky Mountain spotted fever, tularemia, Colorado tick fever and bovine anaplasmosis. Tick saliva is known to modulate both innate and acquired immune responses, enabling ticks to feed for several days without detection. During feeding ticks subvert host defences such as hemostasis and inflammation, which would otherwise result in coagulation, wound repair and rejection of the tick. Molecular characterization of the proteins and pharmacological molecules secreted in tick saliva offers an opportunity to develop tick vaccines as an alternative to the use of acaricides, as well as new anti-inflammatory drugs. We performed proteomics informed by transcriptomics to identify D. andersoni saliva proteins that are secreted during feeding. The transcript data generated a database of 21,797 consensus sequences, which we used to identify 677 proteins secreted in the saliva of D. andersoni ticks fed for 2 and 5 days, following proteomic investigations of whole saliva using mass spectrometry. Salivary gland transcript levels of unfed ticks were compared with 2 and 5 day fed ticks to identify genes upregulated early during tick feeding. We cross-referenced the proteomic data with the transcriptomic data to identify 157 proteins of interest for immunomodulation and blood feeding. Proteins of unknown function as well as known immunomodulators were identified.},
doi = {10.1016/j.ijpara.2014.07.003},
journal = {International Journal for Parasitology, 44(13):1029-1037},
number = ,
volume = ,
place = {United States},
year = {Thu Aug 07 00:00:00 EDT 2014},
month = {Thu Aug 07 00:00:00 EDT 2014}
}
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