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Title: Yeast cell surface display for lipase whole cell catalyst and its applications

Abstract

The cell surface display technique allows for the expression of target proteins or peptides on the microbial cell surface by fusing an appropriate protein as an anchoring motif. Yeast display systems, such as Pichia pastoris, Yarowia lipolytica and Saccharomyces cerevisiae, are ideal, alternative and extensive display systems with the advantage of simple genetic manipulation and post-translational modification of expressed heterologous proteins. Engineered yeasts show high performance characteristics and variant utilizations. Herein, we comprehensively summarize the variant factors affecting lipase whole cell catalyst activity and display efficiency, including the structure and size of target proteins, screening anchor proteins, type and chain length of linkers, and the appropriate matching rules among the above-mentioned display units. Furthermore, we also address novel approaches to enhance stability and activity of recombinant lipases, such as VHb gene co-expression, multi-enzyme co-display technique, and the micro-environmental interference and self-assembly techniques. Finally, we represent the variety of applications of whole cell surface displayed lipases on yeast cells in non-aqueous phases, including synthesis of esters, PUFA enrichment, resolution of chiral drugs, organic synthesis and biofuels. We demonstrate that the lipase surface display technique is a powerful tool for functionalizing yeasts to serve as whole cell catalysts, and increasing interest ismore » providing an impetus for broad application of this technique.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1170471
Report Number(s):
PNNL-SA-102321
47292; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Molecular Catalysis B: Enzymatic, 106:17-25
Country of Publication:
United States
Language:
English
Subject:
Whole cell catalyst; Lipase; Surface display technique; Yeast cell; Application; Environmental Molecular Sciences Laboratory

Citation Formats

Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, and Wright, Aaron T. Yeast cell surface display for lipase whole cell catalyst and its applications. United States: N. p., 2014. Web. doi:10.1016/j.molcatb.2014.04.011.
Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, & Wright, Aaron T. Yeast cell surface display for lipase whole cell catalyst and its applications. United States. doi:10.1016/j.molcatb.2014.04.011.
Liu, Yun, Zhang, Rui, Lian, Zhongshuai, Wang, Shihui, and Wright, Aaron T. Fri . "Yeast cell surface display for lipase whole cell catalyst and its applications". United States. doi:10.1016/j.molcatb.2014.04.011.
@article{osti_1170471,
title = {Yeast cell surface display for lipase whole cell catalyst and its applications},
author = {Liu, Yun and Zhang, Rui and Lian, Zhongshuai and Wang, Shihui and Wright, Aaron T.},
abstractNote = {The cell surface display technique allows for the expression of target proteins or peptides on the microbial cell surface by fusing an appropriate protein as an anchoring motif. Yeast display systems, such as Pichia pastoris, Yarowia lipolytica and Saccharomyces cerevisiae, are ideal, alternative and extensive display systems with the advantage of simple genetic manipulation and post-translational modification of expressed heterologous proteins. Engineered yeasts show high performance characteristics and variant utilizations. Herein, we comprehensively summarize the variant factors affecting lipase whole cell catalyst activity and display efficiency, including the structure and size of target proteins, screening anchor proteins, type and chain length of linkers, and the appropriate matching rules among the above-mentioned display units. Furthermore, we also address novel approaches to enhance stability and activity of recombinant lipases, such as VHb gene co-expression, multi-enzyme co-display technique, and the micro-environmental interference and self-assembly techniques. Finally, we represent the variety of applications of whole cell surface displayed lipases on yeast cells in non-aqueous phases, including synthesis of esters, PUFA enrichment, resolution of chiral drugs, organic synthesis and biofuels. We demonstrate that the lipase surface display technique is a powerful tool for functionalizing yeasts to serve as whole cell catalysts, and increasing interest is providing an impetus for broad application of this technique.},
doi = {10.1016/j.molcatb.2014.04.011},
journal = {Journal of Molecular Catalysis B: Enzymatic, 106:17-25},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 2014},
month = {Fri Aug 01 00:00:00 EDT 2014}
}