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Title: Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle

Abstract

Cyanothece sp. PCC 7822 is an excellent cyanobacterial model organism with great potential to be applied as a biocatalyst for the production of high value compounds. Like other unicellular diazotrophic cyanobacterial species, it has a tightly regulated metabolism synchronized to the light-dark cycle. Utilizing transcriptomic and proteomic methods, we were able to quantify the relationships between transcription and translation underlying central and secondary metabolism in response to nitrogen free, 12 hour light and 12 hour dark conditions.

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1170102
Report Number(s):
PNNL-SA-104956
Journal ID: ISSN 1471-2164; PII: 1471-2164-15-1185
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 15; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; Environmental Molecular Sciences Laboratory

Citation Formats

Welkie, David, Zhang, Xiaohui, Markillie, Meng, Taylor, Ronald, Orr, Galya, Jacobs, Jon, Bhide, Ketaki, Thimmapuram, Jyothi, Gritsenko, Marina, Mitchell, Hugh, Smith, Richard D, and Sherman, Louis A. Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle. United States: N. p., 2014. Web. doi:10.1186/1471-2164-15-1185.
Welkie, David, Zhang, Xiaohui, Markillie, Meng, Taylor, Ronald, Orr, Galya, Jacobs, Jon, Bhide, Ketaki, Thimmapuram, Jyothi, Gritsenko, Marina, Mitchell, Hugh, Smith, Richard D, & Sherman, Louis A. Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle. United States. https://doi.org/10.1186/1471-2164-15-1185
Welkie, David, Zhang, Xiaohui, Markillie, Meng, Taylor, Ronald, Orr, Galya, Jacobs, Jon, Bhide, Ketaki, Thimmapuram, Jyothi, Gritsenko, Marina, Mitchell, Hugh, Smith, Richard D, and Sherman, Louis A. 2014. "Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle". United States. https://doi.org/10.1186/1471-2164-15-1185. https://www.osti.gov/servlets/purl/1170102.
@article{osti_1170102,
title = {Transcriptomic and proteomic dynamics in the metabolism of a diazotrophic cyanobacterium, Cyanothece sp. PCC 7822 during a diurnal light–dark cycle},
author = {Welkie, David and Zhang, Xiaohui and Markillie, Meng and Taylor, Ronald and Orr, Galya and Jacobs, Jon and Bhide, Ketaki and Thimmapuram, Jyothi and Gritsenko, Marina and Mitchell, Hugh and Smith, Richard D and Sherman, Louis A},
abstractNote = {Cyanothece sp. PCC 7822 is an excellent cyanobacterial model organism with great potential to be applied as a biocatalyst for the production of high value compounds. Like other unicellular diazotrophic cyanobacterial species, it has a tightly regulated metabolism synchronized to the light-dark cycle. Utilizing transcriptomic and proteomic methods, we were able to quantify the relationships between transcription and translation underlying central and secondary metabolism in response to nitrogen free, 12 hour light and 12 hour dark conditions.},
doi = {10.1186/1471-2164-15-1185},
url = {https://www.osti.gov/biblio/1170102}, journal = {BMC Genomics},
issn = {1471-2164},
number = 1,
volume = 15,
place = {United States},
year = {Mon Dec 29 00:00:00 EST 2014},
month = {Mon Dec 29 00:00:00 EST 2014}
}

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Cited by: 15 works
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Works referenced in this record:

Proteome Analyses of Strains ATCC 51142 and PCC 7822 of the Diazotrophic Cyanobacterium Cyanothece sp. under Culture Conditions Resulting in Enhanced H 2 Production
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journal, January 2011


Transcriptome and Proteome Dynamics of a Light-Dark Synchronized Bacterial Cell Cycle
journal, August 2012


Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822
journal, October 2013


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journal, November 2006


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journal, January 2007


Dynamic proteomic profiling of a unicellular cyanobacterium Cyanothece ATCC51142 across light-dark diurnal cycles
journal, January 2011


The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
journal, November 2010


Evolution and classification of the CRISPR–Cas systems
journal, May 2011


Genomic analysis of a novel active prophage of Hafnia paralvei
journal, June 2022


Dynamic Proteome Analysis of Cyanothece sp. ATCC 51142 under Constant Light
journal, December 2011


Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008


RNA-Seq: a revolutionary tool for transcriptomics
journal, January 2009


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journal, January 2008


Alternate copies of D1 are used by cyanobacteria under different environmental conditions
journal, November 2012


Fully Automated Four-Column Capillary LC−MS System for Maximizing Throughput in Proteomic Analyses
journal, January 2008


Next generation sequencing of microbial transcriptomes: challenges and opportunities
journal, January 2010


Probability-Based Evaluation of Peptide and Protein Identifications from Tandem Mass Spectrometry and SEQUEST Analysis:  The Human Proteome
journal, February 2005


Dynamic Proteome Analysis of Cyanothece sp. ATCC 51142 under Constant Light
journal, December 2011


Alternative isoleucine synthesis pathway in cyanobacterial species
journal, October 2009


Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
journal, November 2009


Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008


RNA-Seq: a revolutionary tool for transcriptomics
journal, January 2009


Alternate copies of D1 are used by cyanobacteria under different environmental conditions
journal, November 2012


Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
journal, June 2013


Effect of continuous light on diurnal rhythms in Cyanothece sp. ATCC 51142
journal, January 2009


Chemically Etched Open Tubular and Monolithic Emitters for Nanoelectrospray Ionization Mass Spectrometry
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Simple Conditions for Growth of Unicellular Blue-Green Algae on Plates
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Simple Conditions for Growth of Unicellular Blue-Green Algae on Plates
journal, March 1968


Analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822
journal, October 2013


CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats
journal, May 2007


Alternative isoleucine synthesis pathway in cyanobacterial species
journal, October 2009


Works referencing / citing this record:

Iron–Nutrient Interactions within Phytoplankton
journal, August 2016


The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II
journal, May 2016


Iron–Nutrient Interactions within Phytoplankton
journal, August 2016


Metabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions
journal, October 2016


Metabolomic Responses of Arabidopsis Suspension Cells to Bicarbonate under Light and Dark Conditions
journal, October 2016