skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica

Abstract

In order to develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. Finally, the successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism.

Authors:
 [1];  [1];  [1];  [2];  [2];  [1];  [2];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1169214
Report Number(s):
NREL/JA-2700-62665
Journal ID: ISSN 1932-6203
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 9; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; chemical and biosciences; bioenergy; xylanase; xylosidase; Yarrowia lipolytica; heterologous expression; xylan; xylose; digestion; cell cultures; protein expression; saccharomyces cerevisiae; maize; recombinant proteins; secretion

Citation Formats

Wang, Wei, Wei, Hui, Alahuhta, Markus, Chen, Xiaowen, Hyman, Deborah, Johnson, David K., Zhang, Min, and Himmel, Michael E. Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica. United States: N. p., 2014. Web. doi:10.1371/journal.pone.0111443.
Wang, Wei, Wei, Hui, Alahuhta, Markus, Chen, Xiaowen, Hyman, Deborah, Johnson, David K., Zhang, Min, & Himmel, Michael E. Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica. United States. doi:10.1371/journal.pone.0111443.
Wang, Wei, Wei, Hui, Alahuhta, Markus, Chen, Xiaowen, Hyman, Deborah, Johnson, David K., Zhang, Min, and Himmel, Michael E. Tue . "Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica". United States. doi:10.1371/journal.pone.0111443. https://www.osti.gov/servlets/purl/1169214.
@article{osti_1169214,
title = {Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica},
author = {Wang, Wei and Wei, Hui and Alahuhta, Markus and Chen, Xiaowen and Hyman, Deborah and Johnson, David K. and Zhang, Min and Himmel, Michael E.},
abstractNote = {In order to develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. Finally, the successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism.},
doi = {10.1371/journal.pone.0111443},
journal = {PLoS ONE},
number = 12,
volume = 9,
place = {United States},
year = {Tue Dec 02 00:00:00 EST 2014},
month = {Tue Dec 02 00:00:00 EST 2014}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 14 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Interlaboratory testing of methods for assay of xylanase activity
journal, May 1992

  • Bailey, Michael J.; Biely, Peter; Poutanen, Kaisa
  • Journal of Biotechnology, Vol. 23, Issue 3, p. 257-270
  • DOI: 10.1016/0168-1656(92)90074-J

Physiology and genetics of the dimorphic fungus Yarrowia lipolytica
journal, April 1997


Xylanases, xylanase families and extremophilic xylanases
journal, January 2005


Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate
journal, October 2011

  • Tsigie, Yeshitila Asteraye; Wang, Chun-Yuan; Truong, Chi-Thanh
  • Bioresource Technology, Vol. 102, Issue 19, p. 9216-9222
  • DOI: 10.1016/j.biortech.2011.06.047

Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review
journal, April 2004

  • Madzak, Catherine; Gaillardin, Claude; Beckerich, Jean-Marie
  • Journal of Biotechnology, Vol. 109, Issue 1-2, p. 63-81
  • DOI: 10.1016/j.jbiotec.2003.10.027