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Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:
Publication Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167243
Patent Number(s):
8,932,576
Application Number:
12/695,101
Assignee:
The Scripps Research Institute (La Jolla, CA) CHO
DOE Contract Number:
FG03-02ER15313
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Jan 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. United States: N. p., 2015. Web.
Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. United States.
Mayfield, Stephen P. Tue . "Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast". United States. doi:. https://www.osti.gov/servlets/purl/1167243.
@article{osti_1167243,
title = {Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast},
author = {Mayfield, Stephen P},
abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 13 00:00:00 EST 2015},
month = {Tue Jan 13 00:00:00 EST 2015}
}

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