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Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Patent ·
OSTI ID:1167243

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-02ER15313
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,932,576
Application Number:
12/695,101
OSTI ID:
1167243
Resource Relation:
Patent File Date: 2010 Jan 27
Country of Publication:
United States
Language:
English

References (23)

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