Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Tsien, Roger Y; Wang, Lei

Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Patent · 12 January 2015

OSTI ID:1167214

Tsien, Roger Y; Wang, Lei

Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

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Research Organization:: The Regents of the University of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG03-01ER63276

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,932,859

Application Number:: 12/124,888

OSTI ID:: 1167214

Country of Publication:: United States

Language:: English

References (26)

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