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Title: Pyrococcus furiosus strains and methods of using same

Abstract

Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Inventors:
; ; ;
Publication Date:
Research Org.:
University of Georgia Research Foundation, Inc., Athens, GA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167026
Patent Number(s):
8,927,254
Application Number:
13/248,377
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA) ORO
DOE Contract Number:
FG05-95ER20175
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Sep 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., and Westpheling, Janet. Pyrococcus furiosus strains and methods of using same. United States: N. p., 2015. Web.
Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., & Westpheling, Janet. Pyrococcus furiosus strains and methods of using same. United States.
Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., and Westpheling, Janet. Tue . "Pyrococcus furiosus strains and methods of using same". United States. doi:. https://www.osti.gov/servlets/purl/1167026.
@article{osti_1167026,
title = {Pyrococcus furiosus strains and methods of using same},
author = {Lipscomb, Gina L and Farkas, Joel Andrew and Adams, Michael W. W. and Westpheling, Janet},
abstractNote = {Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 06 00:00:00 EST 2015},
month = {Tue Jan 06 00:00:00 EST 2015}
}

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