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Title: High temperature pre-digestion of corn stover biomass for improved product yields

Abstract

Introduction: The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the enzymatic saccharification process step. Here, we describe a novel hybrid processing approach comprising enzymatic pre-digestion with newly characterized hyperthermophilic enzyme cocktails followed by conventional saccharification with commercial enzyme preparations. Dilute acid pretreated corn stover was subjected to this new procedure to test its efficacy. Thermal tolerant enzymes from Acidothermus cellulolyticus and Caldicellulosiruptor bescii were used to pre-digest pretreated biomass at elevated temperatures prior to saccharification by the commercial cellulase formulation. Results: We report that pre-digestion of biomass with these enzymes at elevated temperatures prior to addition of the commercial cellulase formulation increased conversion rates and yields when compared to commercial cellulase formulation alone under low solids conditions. In conclusion, Our results demonstrating improvements in rates and yields of conversion point the way forward for hybrid biomass conversion schemes utilizing catalytic amounts of hyperthermophilic enzymes.

Authors:
 [1];  [1];  [1];  [2];  [2];  [1];  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Chemical Biosciences Center.
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center.
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1166653
Report Number(s):
NREL/JA-2700-62534
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Chemical and Biosciences; Bioenergy; biomass; pretreatment; enzymatic hydrolysis; CelA; E1; Caldicellulosiruptor bescii; Acidothermus cellulolyticus; Thermotoga maritima

Citation Formats

Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., and Decker, Stephen R. High temperature pre-digestion of corn stover biomass for improved product yields. United States: N. p., 2014. Web. doi:10.1186/s13068-014-0170-2.
Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., & Decker, Stephen R. High temperature pre-digestion of corn stover biomass for improved product yields. United States. doi:10.1186/s13068-014-0170-2.
Brunecky, Roman, Hobdey, Sarah E., Taylor, Larry E., Tao, Ling, Tucker, Melvin P., Himmel, Michael E., and Decker, Stephen R. Wed . "High temperature pre-digestion of corn stover biomass for improved product yields". United States. doi:10.1186/s13068-014-0170-2. https://www.osti.gov/servlets/purl/1166653.
@article{osti_1166653,
title = {High temperature pre-digestion of corn stover biomass for improved product yields},
author = {Brunecky, Roman and Hobdey, Sarah E. and Taylor, Larry E. and Tao, Ling and Tucker, Melvin P. and Himmel, Michael E. and Decker, Stephen R.},
abstractNote = {Introduction: The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the enzymatic saccharification process step. Here, we describe a novel hybrid processing approach comprising enzymatic pre-digestion with newly characterized hyperthermophilic enzyme cocktails followed by conventional saccharification with commercial enzyme preparations. Dilute acid pretreated corn stover was subjected to this new procedure to test its efficacy. Thermal tolerant enzymes from Acidothermus cellulolyticus and Caldicellulosiruptor bescii were used to pre-digest pretreated biomass at elevated temperatures prior to saccharification by the commercial cellulase formulation. Results: We report that pre-digestion of biomass with these enzymes at elevated temperatures prior to addition of the commercial cellulase formulation increased conversion rates and yields when compared to commercial cellulase formulation alone under low solids conditions. In conclusion, Our results demonstrating improvements in rates and yields of conversion point the way forward for hybrid biomass conversion schemes utilizing catalytic amounts of hyperthermophilic enzymes.},
doi = {10.1186/s13068-014-0170-2},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Wed Dec 03 00:00:00 EST 2014},
month = {Wed Dec 03 00:00:00 EST 2014}
}

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Cited by: 3 works
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Works referenced in this record:

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journal, October 2000

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Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA
journal, December 2013