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Title: Pnp gene modification for improved xylose utilization in Zymomonas

Abstract

The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
E I Du Pont de Nemours and Company, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1165440
Patent Number(s):
8,911,983
Application Number:
13/711,646
Assignee:
E I Du Pont de Nemours and Company (Wilmington, DE) GFO
DOE Contract Number:  
FC36-07GO17056
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Dec 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States: N. p., 2014. Web.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, & Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Tue . "Pnp gene modification for improved xylose utilization in Zymomonas". United States. https://www.osti.gov/servlets/purl/1165440.
@article{osti_1165440,
title = {Pnp gene modification for improved xylose utilization in Zymomonas},
author = {Caimi, Perry G G and Qi, Min and Tao, Luan and Viitanen, Paul V and Yang, Jianjun},
abstractNote = {The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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Works referenced in this record:

Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
journal, February 2005


Global analysis of Escherichia coli RNA degradosome function using DNA microarrays
journal, February 2004

  • Bernstein, J. A.; Lin, P.-H.; Cohen, S. N.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 9, p. 2758-2763
  • DOI: 10.1073/pnas.0308747101