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Title: Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid

Abstract

Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.

Inventors:
; ;
Publication Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1164688
Patent Number(s):
8,900,835
Application Number:
13/301,836
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG36-04GO14019
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Nov 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States: N. p., 2014. Web.
Wang, Qingzhao, Shanmugam, Keelnatham T, & Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States.
Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Tue . "Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid". United States. https://www.osti.gov/servlets/purl/1164688.
@article{osti_1164688,
title = {Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid},
author = {Wang, Qingzhao and Shanmugam, Keelnatham T and Ingram, Lonnie O},
abstractNote = {Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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Works referenced in this record:

Metabolic Flux Control at the Pyruvate Node in an Anaerobic Escherichia coli Strain with an Active Pyruvate Dehydrogenase
journal, January 2010

  • Wang, Q.; Ou, M. S.; Kim, Y.
  • Applied and Environmental Microbiology, Vol. 76, Issue 7, p. 2107-2114
  • DOI: 10.1128/AEM.02545-09

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Metabolic Engineering of Bacillus subtilis for Ethanol Production: Lactate Dehydrogenase Plays a Key Role in Fermentative Metabolism
journal, June 2007

  • Romero, S.; Merino, E.; Bolivar, F.
  • Applied and Environmental Microbiology, Vol. 73, Issue 16, p. 5190-5198
  • DOI: 10.1128/AEM.00625-07

Physiological and fermentation properties of Bacillus coagulans and a mutant lacking fermentative lactate dehydrogenase activity
journal, July 2010

  • Su, Yue; Rhee, Mun Su; Ingram, Lonnie O.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 3
  • DOI: 10.1007/s10295-010-0788-4

One-step production of lactate from cellulose as the sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis
journal, July 2011

  • Zhang, Xiao-Zhou; Sathitsuksanoh, Noppadon; Zhu, Zhiguang
  • Metabolic Engineering, Vol. 13, Issue 4, p. 364-372
  • DOI: 10.1016/j.ymben.2011.04.003

Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose
journal, November 2011

  • Wang, Q.; Ingram, L. O.; Shanmugam, K. T.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 47, p. 18920-18925
  • DOI: 10.1073/pnas.1111085108

Lactic acid: recent advances in products, processes and technologies — a review
journal, January 2006

  • Datta, Rathin; Henry, Michael
  • Journal of Chemical Technology & Biotechnology, Vol. 81, Issue 7, p. 1119-1129
  • DOI: 10.1002/jctb.1486

Methylglyoxal Bypass Identified as Source of Chiral Contamination in l(+) and d(−)-lactate Fermentations by Recombinant Escherichia coli
journal, July 2006


Factors affecting the fermentative lactic acid production from renewable resources
journal, February 2000


Construction of an Escherichia coli K-12 Mutant for Homoethanologenic Fermentation of Glucose or Xylose without Foreign Genes
journal, January 2007

  • Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
  • Applied and Environmental Microbiology, Vol. 73, Issue 6, p. 1766-1771
  • DOI: 10.1128/AEM.02456-06

Dihydrolipoamide Dehydrogenase Mutation Alters the NADH Sensitivity of Pyruvate Dehydrogenase Complex of Escherichia coli K-12
journal, March 2008

  • Kim, Y.; Ingram, L. O.; Shanmugam, K. T.
  • Journal of Bacteriology, Vol. 190, Issue 11, p. 3851-3858
  • DOI: 10.1128/JB.00104-08

l(+)-Lactic acid production from non-food carbohydrates by thermotolerant Bacillus coagulans
journal, August 2010

  • Ou, Mark S.; Ingram, Lonnie O.; Shanmugam, K. T.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 5
  • DOI: 10.1007/s10295-010-0796-4

Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid
journal, May 2006


Lactic acid production by Bacillus coagulans—kinetic studies and optimization of culture medium for batch and continuous fermentations
journal, December 1998


Genetic Changes To Optimize Carbon Partitioning between Ethanol and Biosynthesis in Ethanologenic Escherichia coli
journal, December 2002


Functional Replacement of the Escherichia coliD-(-)-Lactate Dehydrogenase Gene (ldhA) with the L-(+)-Lactate Dehydrogenase Gene (ldhL) from Pediococcus acidilactici
journal, April 2003


Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae
journal, November 2011

  • Oh, Baek-Rock; Seo, Jeong-Woo; Heo, Sun-Yeon
  • Applied Biochemistry and Biotechnology, Vol. 166, Issue 1
  • DOI: 10.1007/s12010-011-9409-6