Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Vertes, Akos; Stolee, Jessica A; Shrestha, Bindesh

Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Patent · 02 December 2014

OSTI ID:1164685

Vertes, Akos; Stolee, Jessica A; Shrestha, Bindesh

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

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Research Organization:: George Washington University, Washington, DC (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-01ER15129

Assignee:: George Washington University (Washington, DC)

Patent Number(s):: 8,901,487

Application Number:: 13/045,277

OSTI ID:: 1164685

Country of Publication:: United States

Language:: English

References (21)

Infrared laser-assisted desorption electrospray ionization mass spectrometry Rezenom, Yohannes H.; Dong, Jianan; Murray, Kermit K. The Analyst, Vol. 133, Issue 2 https://doi.org/10.1039/B715146B	journal	January 2008
Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions Cody, Robert B.; Laramée, James A.; Durst, H. Dupont Analytical Chemistry, Vol. 77, Issue 8, p. 2297-2302 https://doi.org/10.1021/ac050162j	journal	April 2005
Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization Takats, Z. Science, Vol. 306, Issue 5695, p. 471-473 https://doi.org/10.1126/science.1104404	journal	October 2004
New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy Rasmussen, Akiko; Deckert, Volker Analytical and Bioanalytical Chemistry, Vol. 381, Issue 1 https://doi.org/10.1007/s00216-004-2896-3	journal	November 2004
Simultaneous Imaging of Small Metabolites and Lipids in Rat Brain Tissues at Atmospheric Pressure by Laser Ablation Electrospray Ionization Mass Spectrometry Nemes, Peter; Woods, Amina S.; Vertes, Akos Analytical Chemistry, Vol. 82, Issue 3 https://doi.org/10.1021/ac902245p	journal	February 2010
Direct Protein Detection from Biological Media through Electrospray-Assisted Laser Desorption Ionization/Mass Spectrometry Huang, Min-Zong; Hsu, Hsiu-Jung; Lee, Jen-Yih Journal of Proteome Research, Vol. 5, Issue 5 https://doi.org/10.1021/pr050442f	journal	May 2006
Matrix-assisted laser desorption and ionization in the OH and CO absorption bands of aliphatic and aromatic matrices: dependence on laser wavelength and temporal beam profile Cramer, Rainer; Haglund, Richard F.; Hillenkamp, Franz International Journal of Mass Spectrometry and Ion Processes, Vol. 169-170, p. 51-67 https://doi.org/10.1016/S0168-1176(97)00223-1	journal	December 1997
Nanoscale Atmospheric Pressure Laser Ablation-Mass Spectrometry Stöckle, Raoul; Setz, Patrick; Deckert, Volker Analytical Chemistry, Vol. 73, Issue 7 https://doi.org/10.1021/ac001440b	journal	April 2001
Elevated subcortical choline metabolites in cognitively and clinically asymptomatic HIV patients Meyerhoff, D. J.; Bloomer, C.; Cardenas, V. Neurology, Vol. 52, Issue 5 https://doi.org/10.1212/WNL.52.5.995	journal	March 1999
Infrared Laser Ablation Atmospheric Pressure Photoionization Mass Spectrometry Vaikkinen, Anu; Shrestha, Bindesh; Kauppila, Tiina J. Analytical Chemistry, Vol. 84, Issue 3 https://doi.org/10.1021/ac202905y	journal	January 2012
Ablation and analysis of small cell populations and single cells by consecutive laser pulses Shrestha, Bindesh; Nemes, Peter; Vertes, Akos Applied Physics A, Vol. 101, Issue 1 https://doi.org/10.1007/s00339-010-5781-2	journal	June 2010
Three-Dimensional Imaging of Metabolites in Tissues under Ambient Conditions by Laser Ablation Electrospray Ionization Mass Spectrometry Nemes, Peter; Barton, Alexis A.; Vertes, Akos Analytical Chemistry, Vol. 81, Issue 16 https://doi.org/10.1021/ac900745e	journal	August 2009
Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry Sampson, Jason S.; Murray, Kermit K.; Muddiman, David C. Journal of the American Society for Mass Spectrometry, Vol. 20, Issue 4, p. 667-673 https://doi.org/10.1016/j.jasms.2008.12.003	journal	April 2009
Laser Desorption-Atmospheric Pressure Chemical Ionization Mass Spectrometry for the Analysis of Peptides from Aqueous Solutions Coon, Joshua J.; Harrison, W. W. Analytical Chemistry, Vol. 74, Issue 21 https://doi.org/10.1021/ac020402k	journal	November 2002
Ambient Molecular Imaging and Depth Profiling of Live Tissue by Infrared Laser Ablation Electrospray Ionization Mass Spectrometry Nemes, Peter; Barton, Alexis A.; Li, Yue Analytical Chemistry, Vol. 80, Issue 12 https://doi.org/10.1021/ac8004082	journal	June 2008
Free-electron-laser-based biophysical and biomedical instrumentation Edwards, G. S.; Austin, R. H.; Carroll, F. E. Review of Scientific Instruments, Vol. 74, Issue 7 https://doi.org/10.1063/1.1584078	journal	July 2003
FT-IR imaging of native and tissue-engineered bone and cartilage Boskey, A.; Pleshkocamacho, N. Biomaterials, Vol. 28, Issue 15 https://doi.org/10.1016/j.biomaterials.2006.11.043	journal	May 2007
Recent Advances in Optical Tweezers Moffitt, Jeffrey R.; Chemla, Yann R.; Smith, Steven B. Annual Review of Biochemistry, Vol. 77, Issue 1 https://doi.org/10.1146/annurev.biochem.77.043007.090225	journal	June 2008
Laser Capture Microdissection Emmert-Buck, Michael R.; Bonner, Robert F.; Smith, Paul D. Science, Vol. 274, Issue 5289 https://doi.org/10.1126/science.274.5289.998	journal	November 1996
In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry Shrestha, Bindesh; Vertes, Akos Analytical Chemistry, Vol. 81, Issue 20 https://doi.org/10.1021/ac901525g	journal	October 2009
Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry Nemes, Peter; Vertes, Akos Analytical Chemistry, Vol. 79, Issue 21 https://doi.org/10.1021/ac071181r	journal	November 2007

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