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Title: Sub-micrometer fluidic channel for measuring photon emitting entities

Abstract

A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

Inventors:
; ; ;
Publication Date:
Research Org.:
Cornell Research Foundation, Ithaca, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1163985
Patent Number(s):
8,890,323
Application Number:
12/716,087
Assignee:
Cornell Research Foundation (Ithaca, NY) CHO
DOE Contract Number:  
FG02-99ER62809
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 36 MATERIALS SCIENCE

Citation Formats

Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, and Craighead, Harold G. Sub-micrometer fluidic channel for measuring photon emitting entities. United States: N. p., 2014. Web.
Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, & Craighead, Harold G. Sub-micrometer fluidic channel for measuring photon emitting entities. United States.
Stavis, Samuel M, Edel, Joshua B, Samiee, Kevan T, and Craighead, Harold G. Tue . "Sub-micrometer fluidic channel for measuring photon emitting entities". United States. doi:. https://www.osti.gov/servlets/purl/1163985.
@article{osti_1163985,
title = {Sub-micrometer fluidic channel for measuring photon emitting entities},
author = {Stavis, Samuel M and Edel, Joshua B and Samiee, Kevan T and Craighead, Harold G},
abstractNote = {A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 18 00:00:00 EST 2014},
month = {Tue Nov 18 00:00:00 EST 2014}
}

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Works referenced in this record:

A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy
journal, January 2003


Autofluorescent Proteins in Single-Molecule Research: Applications to Live Cell Imaging Microscopy
journal, May 2001

  • Harms, Gregory S.; Cognet, Laurent; Lommerse, Piet H. M.
  • Biophysical Journal, Vol. 80, Issue 5, p. 2396-2408
  • DOI: 10.1016/S0006-3495(01)76209-1

In Vivo Multiphoton Microscopy of Deep Brain Tissue
journal, April 2004

  • Levene, Michael J.; Dombeck, Daniel A.; Kasischke, Karl A.
  • Journal of Neurophysiology, Vol. 91, Issue 4, p. 1908-1912
  • DOI: 10.1152/jn.01007.2003

Optical coding of mammalian cells using semiconductor quantum dots
journal, April 2004

  • Mattheakis, Larry C.; Dias, Jennifer M.; Choi, Yun-Jung
  • Analytical Biochemistry, Vol. 327, Issue 2, p. 200-208
  • DOI: 10.1016/j.ab.2004.01.031

Factors that influence confocal apertureless near-field scanning optical microscopy
journal, March 2004

  • Protasenko, Vladimir V.; Gallagher, Alan; Nesbitt, David J.
  • Optics Communications, Vol. 233, Issue 1-3, p. 45-56
  • DOI: 10.1016/j.optcom.2004.01.004

Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution
journal, April 1997