Sub-micrometer fluidic channel for measuring photon emitting entities

Stavis, Samuel M; Edel, Joshua B; Samiee, Kevan T; Craighead, Harold G

Sub-micrometer fluidic channel for measuring photon emitting entities

Patent · 18 November 2014

OSTI ID:1163985

Stavis, Samuel M; Edel, Joshua B; Samiee, Kevan T; Craighead, Harold G

A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

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Research Organization:: Cornell Research Foundation, Ithaca, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-99ER62809

Assignee:: Cornell Research Foundation (Ithaca, NY)

Patent Number(s):: 8,890,323

Application Number:: 12/716,087

OSTI ID:: 1163985

Country of Publication:: United States

Language:: English

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