U.S. Department of EnergyOffice of Scientific and Technical Information
Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings
Abstract
To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.
- Authors:
-
- Univ. of South Carolina, Aiken, SC (United States)
- Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
- North Carolina State Univ., Raleigh, NC (United States)
- Publication Date:
- Research Org.:
- Univ. of South Carolina, Columbia, SC (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; USDOE Office of Environmental Management (EM)
- OSTI Identifier:
- 1163478
- Report Number(s):
- DOE-USCA-3152-2
Journal ID: ISSN 1751-7915
- Grant/Contract Number:
- EE0003152
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Microbial Biotechnology (Online)
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 5; Journal ID: ISSN 1751-7915
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS
Citation Formats
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States: N. p., 2013.
Web. doi:10.1111/1751-7915.12032.
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., & Yeager, C. M. Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings. United States. https://doi.org/10.1111/1751-7915.12032
Piskorska, M., Soule, T., Gosse, J. L., Milliken, C., Flickinger, M. C., Smith, G. W., and Yeager, C. M. 2013.
"Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings". United States. https://doi.org/10.1111/1751-7915.12032. https://www.osti.gov/servlets/purl/1163478.
@article{osti_1163478,
title = {Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings},
author = {Piskorska, M. and Soule, T. and Gosse, J. L. and Milliken, C. and Flickinger, M. C. and Smith, G. W. and Yeager, C. M.},
abstractNote = {To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O2 on preservation of H2 production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H2 production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H2 production activity, whereas considerable H2 production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H2 production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H2 (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H2 production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H2 production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.},
doi = {10.1111/1751-7915.12032},
url = {https://www.osti.gov/biblio/1163478},
journal = {Microbial Biotechnology (Online)},
issn = {1751-7915},
number = 5,
volume = 6,
place = {United States},
year = {Sun Jul 21 00:00:00 EDT 2013},
month = {Sun Jul 21 00:00:00 EDT 2013}
}
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Works referencing / citing this record:
The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities
journal, October 2019
- Luo, Luyun; Wang, Pei; Zhai, Zhongying
- AMB Express, Vol. 9, Issue 1
The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities
journal, October 2019
- Luo, Luyun; Wang, Pei; Zhai, Zhongying
- AMB Express, Vol. 9, Issue 1