skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Transferring Biomarker into Molecular Probe: Melanin Nanoparticle as a Naturally Active Platform for Multimodality Imaging

Journal Article · · Journal of the American Chemical Society
DOI:https://doi.org/10.1021/ja505412p· OSTI ID:1162348
 [1];  [2];  [2];  [2];  [2];  [2];  [3];  [4];  [3];  [2];  [2]
  1. Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, Stanford, California 94305-5484, United States, Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing 210046, China
  2. Molecular Imaging Program at Stanford (MIPS), Canary Center at Stanford for Cancer Early Detection, Department of Radiology and Bio-X Program, School of Medicine, Stanford University, Stanford, California 94305-5484, United States
  3. Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing 210046, China
  4. Department of Radiation Oncology, School of Medicine, Stanford University, Stanford, California 94305, United States
+ Show Author Affiliations

Developing multifunctional and easily prepared nanoplatforms with integrated different modalities is highly challenging for molecular imaging. Here, we report the successful transfer of an important molecular target, melanin, into a novel multimodality imaging nanoplatform. Melanin is abundantly expressed in melanotic melanomas and thus has been actively studied as a target for melanoma imaging. In our work, the multifunctional biopolymer nanoplatform based on ultrasmall (<10 nm) water-soluble melanin nanoparticle (MNP) was developed and showed unique photoacoustic property and natural binding ability with metal ions (for example, 64Cu2+, Fe3+). Therefore, MNP can serve not only as a photoacoustic contrast agent, but also as a nanoplatform for positron emission tomography (PET) and magnetic resonance imaging (MRI). Traditional passive nanoplatforms require complicated and time-consuming processes for prebuilding reporting moieties or chemical modifications using active groups to integrate different contrast properties into one entity. In comparison, utilizing functional biomarker melanin can greatly simplify the building process. We further conjugated αvβ3 integrins, cyclic c(RGDfC) peptide, to MNPs to allow for U87MG tumor accumulation due to its targeting property combined with the enhanced permeability and retention (EPR) effect. As a result, the multimodal properties of MNPs demonstrate the high potential of endogenous materials with multifunctions as nanoplatforms for molecular theranostics and clinical translation.

Research Organization:
Stanford Univ., CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
SC0008397
OSTI ID:
1162348
Alternate ID(s):
OSTI ID: 1345806
Journal Information:
Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Vol. 136 Journal Issue: 43; ISSN 0002-7863
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 274 works
Citation information provided by
Web of Science

References (41)

Intracerebral malignant melanoma: high-field-strength MR imaging. journal October 1987
Self-assembled DNA nanostructures for distance-dependent multivalent ligand–protein binding journal June 2008
Synthesis of Conjugated−Ionic Block Copolymers by Controlled Radical Polymerization journal December 2002
Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents journal March 2011
Small-Animal PET of Melanocortin 1 Receptor Expression Using a 18F-Labeled  -Melanocyte-Stimulating Hormone Analog journal June 2007
Radiolabeled Affibody−Albumin Bioconjugates for HER2 -Positive Cancer Targeting journal March 2011
Building plasmonic nanostructures with DNA journal April 2011
Imaging in the era of molecular oncology journal April 2008
Insights into Melanosomes and Melanin from Some Interesting Spatial and Temporal Properties journal September 2008
Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy journal January 2006
Dextran-Coated Iron Oxide Nanoparticles: A Versatile Platform for Targeted Molecular Imaging, Molecular Diagnostics, and Therapy journal October 2011
Radioluminescent Gold Nanocages with Controlled Radioactivity for Real-Time in Vivo Imaging journal January 2013
A Chelator-Free Multifunctional [ 64 Cu]CuS Nanoparticle Platform for Simultaneous Micro-PET/CT Imaging and Photothermal Ablation Therapy journal November 2010
Ion-Exchange and Adsorption of Fe(III) by Sepia Melanin journal June 2004
Intracranial Lesions with High Signal Intensity on T1-weighted MR Images: Differential Diagnosis journal March 2012
Tyrosinase as a multifunctional reporter gene for Photoacoustic/MRI/PET triple modality molecular imaging journal March 2013
Melanins, Melanogenesis and Melanocytes: Looking at Their Functional Significance from the Chemist's Viewpoint: Melanin Chemistry journal August 2000
In Vivo Biodistribution and Small Animal PET of 64 Cu-Labeled Antimicrobial Peptoids journal April 2012
Synthetic nanoparticles functionalized with biomimetic leukocyte membranes possess cell-like functions journal December 2012
64Cu-Labeled Affibody Molecules for Imaging of HER2 Expressing Tumors journal September 2009
High-Relaxivity MRI Contrast Agents: Where Coordination Chemistry Meets Medical Imaging journal October 2008
Carbon nanotubes as photoacoustic molecular imaging agents in living mice journal August 2008
Mussel Inspired Dynamic Cross-Linking of Self-Healing Peptide Nanofiber Network journal November 2012
Multistage Nanovectors: From Concept to Novel Imaging Contrast Agents and Therapeutics journal October 2011
Biopolymers as a Flexible Resource for Nanochemistry journal December 2012
Current Understanding of the Binding Sites, Capacity, Affinity, and Biological Significance of Metals in Melanin journal July 2007
Inherently Multimodal Nanoparticle-Driven Tracking and Real-Time Delineation of Orthotopic Prostate Tumors and Micrometastases journal April 2013
Fiber-laser-based photoacoustic microscopy and melanoma cell detection journal January 2011
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics journal January 2005
Porphyrin Shell Microbubbles with Intrinsic Ultrasound and Photoacoustic Properties journal August 2012
Melanin-Targeted Preclinical PET Imaging of Melanoma Metastasis journal September 2009
Vaccinia virus-mediated melanin production allows MR and optoacoustic deep tissue imaging and laser-induced thermotherapy of cancer journal February 2013
The Melanin Binding of Drugs and Its Implications journal January 1984
Role of Tyrosinase as the Determinant of Pigmentation in Cultured Human Melanocytes journal June 1993
Melanin photoprotection in the human retinal pigment epithelium and its correlation with light-induced cell apoptosis journal June 2005
Mussel-Inspired Surface Chemistry for Multifunctional Coatings journal October 2007
Photoacoustic microscopy of tyrosinase reporter gene in vivo journal January 2011
Non-Covalent Self-Assembly and Covalent Polymerization Co-Contribute to Polydopamine Formation journal July 2012
Family of Enhanced Photoacoustic Imaging Agents for High-Sensitivity and Multiplexing Studies in Living Mice journal May 2012
Three-Dimensional Structures Self-Assembled from DNA Bricks journal November 2012
A review of NIR dyes in cancer targeting and imaging journal October 2011

Similar Records

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
60 APPLIED LIFE SCIENCES