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Title: Process and genes for expression and overexpression of active [FeFe] hydrogenases

Abstract

A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1159827
Patent Number(s):
8,835,153
Application Number:
11/573,035
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO) GFO
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, and Smolinski, Sharon L. Process and genes for expression and overexpression of active [FeFe] hydrogenases. United States: N. p., 2014. Web.
Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, & Smolinski, Sharon L. Process and genes for expression and overexpression of active [FeFe] hydrogenases. United States.
Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, and Smolinski, Sharon L. Tue . "Process and genes for expression and overexpression of active [FeFe] hydrogenases". United States. doi:. https://www.osti.gov/servlets/purl/1159827.
@article{osti_1159827,
title = {Process and genes for expression and overexpression of active [FeFe] hydrogenases},
author = {Seibert, Michael and King, Paul W and Ghirardi, Maria Lucia and Posewitz, Matthew C and Smolinski, Sharon L},
abstractNote = {A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 16 00:00:00 EDT 2014},
month = {Tue Sep 16 00:00:00 EDT 2014}
}

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Works referenced in this record:

Classification and phylogeny of hydrogenases
journal, August 2001


Maturation of the [NiFe] hydrogenases
journal, May 2001