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Title: Process and genes for expression and overexpression of active [FeFe] hydrogenases

Patent ·
OSTI ID:1159827
; ; ; ;

A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,835,153
Application Number:
11/573,035
OSTI ID:
1159827
Resource Relation:
Patent File Date: 2006 Feb 28
Country of Publication:
United States
Language:
English

References (16)

Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli journal January 1987
Methods and Compositions for Evolving Hydrogenase Genes patent-application October 2004
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase journal April 2004
Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12. journal August 1989
Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer journal February 2003
Characterization of a [2Fe-2S] protein encoded in the iron-hydrogenase operon of Thermotoga maritima journal February 2003
Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System journal March 2006
Heterologous expression of clostridial hydrogenase in the cyanobacterium Synechococcus PCC7942 journal February 2000
Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coli journal December 1987
Classification and phylogeny of hydrogenases journal August 2001
High-frequency nuclear transformation of Chlamydomonas reinhardtii. journal February 1990
Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii journal June 1993
Incorporation of Iron and Sulfur from NifB Cofactor into the Iron-Molybdenum Cofactor of Dinitrogenase journal November 1995
Hydrogen production patent February 2005
Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions: Expression of a second [Fe]-hydrogenase in C. reinhardtii journal June 2003
Mutational analysis and characterization of the Escherichia coli hya operon, which encodes [NiFe] hydrogenase 1. journal August 1991

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59 BASIC BIOLOGICAL SCIENCES