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Title: Physiological Stress Responses to Prolonged Exposure to MS-222 and Surgical Implantation in Juvenile Chinook Salmon

Abstract

While many studies have investigated the effects of transmitters on fish condition, behavior, and survival, to our knowledge, no studies have taken into account anesthetic exposure time in addition to tag and surgery effects. We investigated stress responses to prolonged MS-222 exposure after stage 4 induction in surgically implanted juvenile Chinook salmon (Oncorhynchus tshawytscha). Survival, tag loss, plasma cortisol concentration, and blood Na +, K +, Ca 2+, and pH were measured immediately following anesthetic exposure and surgical implantation and 1, 7, and 14 days post-treatment. Despite the prolonged anesthetic exposure, 3-15 minutes post Stage 4 induction, there were no mortalities or tag loss in any treatment. MS-222 was effective at delaying immediate cortisol release during surgical implantation; however, osmotic disturbances resulted, which were more pronounced in longer anesthetic time exposures. From day 1 to day 14, Na +, Ca 2+, and pH significantly decreased, while cortisol significantly increased. The cortisol increase was exacerbated by surgical implantation. There was a significant interaction between MS-222 time exposure and observation day for Na +, Ca 2+, K +, and pH; variations were seen in the longer time exposures, although not consistently. In conclusion, stress response patterns suggest stress associated with surgical implantationmore » is amplified with increased exposure to MS-222.« less

Authors:
 [1];  [1];  [2];  [2];  [3]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Washington, Seattle, WA (United States)
  3. U.S. Army Corps of Engineers, Portland, OR (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1158486
Report Number(s):
PNNL-SA-93651
Journal ID: ISSN 0275-5947; 400403209
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: North American Journal of Fisheries Management; Journal Volume: 34; Journal Issue: 4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Analytes; Anesthesia; Cortisol; MS-222; Stress; Surgical Implantation

Citation Formats

Wagner, Katie A., Woodley, Christa M., Seaburg, Adam, Skalski, John R., and Eppard, Matthew B.. Physiological Stress Responses to Prolonged Exposure to MS-222 and Surgical Implantation in Juvenile Chinook Salmon. United States: N. p., 2014. Web. doi:10.1080/02755947.2014.926303.
Wagner, Katie A., Woodley, Christa M., Seaburg, Adam, Skalski, John R., & Eppard, Matthew B.. Physiological Stress Responses to Prolonged Exposure to MS-222 and Surgical Implantation in Juvenile Chinook Salmon. United States. doi:10.1080/02755947.2014.926303.
Wagner, Katie A., Woodley, Christa M., Seaburg, Adam, Skalski, John R., and Eppard, Matthew B.. Thu . "Physiological Stress Responses to Prolonged Exposure to MS-222 and Surgical Implantation in Juvenile Chinook Salmon". United States. doi:10.1080/02755947.2014.926303.
@article{osti_1158486,
title = {Physiological Stress Responses to Prolonged Exposure to MS-222 and Surgical Implantation in Juvenile Chinook Salmon},
author = {Wagner, Katie A. and Woodley, Christa M. and Seaburg, Adam and Skalski, John R. and Eppard, Matthew B.},
abstractNote = {While many studies have investigated the effects of transmitters on fish condition, behavior, and survival, to our knowledge, no studies have taken into account anesthetic exposure time in addition to tag and surgery effects. We investigated stress responses to prolonged MS-222 exposure after stage 4 induction in surgically implanted juvenile Chinook salmon (Oncorhynchus tshawytscha). Survival, tag loss, plasma cortisol concentration, and blood Na+, K+, Ca2+, and pH were measured immediately following anesthetic exposure and surgical implantation and 1, 7, and 14 days post-treatment. Despite the prolonged anesthetic exposure, 3-15 minutes post Stage 4 induction, there were no mortalities or tag loss in any treatment. MS-222 was effective at delaying immediate cortisol release during surgical implantation; however, osmotic disturbances resulted, which were more pronounced in longer anesthetic time exposures. From day 1 to day 14, Na+, Ca2+, and pH significantly decreased, while cortisol significantly increased. The cortisol increase was exacerbated by surgical implantation. There was a significant interaction between MS-222 time exposure and observation day for Na+, Ca2+, K+, and pH; variations were seen in the longer time exposures, although not consistently. In conclusion, stress response patterns suggest stress associated with surgical implantation is amplified with increased exposure to MS-222.},
doi = {10.1080/02755947.2014.926303},
journal = {North American Journal of Fisheries Management},
number = 4,
volume = 34,
place = {United States},
year = {Thu Jul 17 00:00:00 EDT 2014},
month = {Thu Jul 17 00:00:00 EDT 2014}
}