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Nucleic acid compositions and the encoding proteins

Patent ·
OSTI ID:1154933
The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.
Research Organization:
University of Florida, Gainesville, FL (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FC36-99GO10476; FC36-00GO10594
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Number(s):
8,822,179
Application Number:
13/350,858
OSTI ID:
1154933
Country of Publication:
United States
Language:
English

References (3)

Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4 journal February 2000
Bacteria engineered for fuel ethanol production: current status journal December 2003
Microbial Strategies for the Depolymerization of Glucuronoxylan: Leads to Biotechnological Applications of Endoxylanases book August 2003