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Title: High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery

Abstract

A high-throughput protein-expression and screening method (HIGH method, see picture) provides a rapid approach to the discovery of active glycoside hydrolases in environmental samples. Finally, HIGH screening combines cloning, protein expression, and enzyme hydrolysis in one pot; thus, the entire process from gene expression to activity detection requires only three hours.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Joint Genome Institute (JGI)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1153466
Resource Type:
Journal Article
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Volume: 50; Journal Issue: 47; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Country of Publication:
United States
Language:
English

Citation Formats

Kim, Tae-Wan, Chokhawala, Harshal A., Hess, Matthias, Dana, Craig M., Baer, Zachary, Sczyrba, Alexander, Rubin, Edward M., Blanch, Harvey W., and Clark, Douglas S. High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery. United States: N. p., 2011. Web. doi:10.1002/anie.201104685.
Kim, Tae-Wan, Chokhawala, Harshal A., Hess, Matthias, Dana, Craig M., Baer, Zachary, Sczyrba, Alexander, Rubin, Edward M., Blanch, Harvey W., & Clark, Douglas S. High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery. United States. doi:10.1002/anie.201104685.
Kim, Tae-Wan, Chokhawala, Harshal A., Hess, Matthias, Dana, Craig M., Baer, Zachary, Sczyrba, Alexander, Rubin, Edward M., Blanch, Harvey W., and Clark, Douglas S. Fri . "High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery". United States. doi:10.1002/anie.201104685.
@article{osti_1153466,
title = {High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery},
author = {Kim, Tae-Wan and Chokhawala, Harshal A. and Hess, Matthias and Dana, Craig M. and Baer, Zachary and Sczyrba, Alexander and Rubin, Edward M. and Blanch, Harvey W. and Clark, Douglas S.},
abstractNote = {A high-throughput protein-expression and screening method (HIGH method, see picture) provides a rapid approach to the discovery of active glycoside hydrolases in environmental samples. Finally, HIGH screening combines cloning, protein expression, and enzyme hydrolysis in one pot; thus, the entire process from gene expression to activity detection requires only three hours.},
doi = {10.1002/anie.201104685},
journal = {Angewandte Chemie (International Edition)},
issn = {1433-7851},
number = 47,
volume = 50,
place = {United States},
year = {2011},
month = {9}
}